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M.T. da Rocha Lima1, O. Custódio2, P. Ferreira do Prado Moreira3, L.M. Quirino Araujo2, C. de Mello Almada Filho2, M. Seabra Cendoroglo4


1. Geriatrics Division, Paulista School of Medicine, Federal University of Sao Paulo, Brazil; 2. Geriatrics Division, Paulista School of Medicine, Federal University of Sao Paulo, Brazil; 3. Nutritionist at the Geriatrics Division, Paulista School of Medicine, Federal University of Sao Paulo, Brazil; 4. Professor at the Geriatrics Division, Paulista School of Medicine, Federal University of Sao Paulo, Brazil

Corresponding Author: Márcio Tomita da Rocha Lima, Rua Professor Francisco de Castro, 105, Vila Clementino, CEP 04020-050, São Paulo – São Paulo, Brazil, Telephone number: +551155764848 – extension line 2298, marciotrl@yahoo.com.br

J Aging Res Clin Practice 2016;5(3):142-146
Published online June 16, 2016, http://dx.doi.org/10.14283/jarcp.2016.101



Background: Nowadays, the relation between hypovitaminosis D and depression has been reported and it is estimated that 1 billion people worldwide have vitamin D deficiency or insufficiency. However, the oldest old people are not included or are under-represented in most of the studies. Objective: To examine the association between depressive symptoms and 25-hydroxyvitamin D level (25(OH)Vit D) in elderly aged 80 and over who are physically more active and independent. Design: Cross-sectional study. Setting and Participants: Data collected from 182 oldest old people, aged 80 and over in the Geriatric Division from Federal University of São Paulo. Measurements: The functionality was evaluated by the Instrumental activities of their daily living (IADL). The approach of the depressive symptoms was done by the Geriatric Depression Scale (GDS) in its reduced 15 item version. 25-hydroxyvitamin D (25(OH)Vit D) analyses was done in serum sample refrigerated and protected from solar exposition. We considered deficiency serum level of 25(OH)Vit D <10ng/mL, insufficiency between 10 and 30ng/mL and sufficiency >30ng/mL. Results: According to blood level of 25(OH)Vit D we found difference between GDS score comparing the groups: “deficiency” (U=144,50; z=-3,126; p=0,002) and “insufficiency” groups (U=975,50; z=-2,793; p=0.005) are different than “sufficiency” group. Conclusion: In free-living independent oldest old people the goal of 25(OH)Vit D levels can be higher to avoid depressive symptoms, levels under 30ng/mL can be inadequate. Considering that the costs are low and side effects are not common, 25(OH)Vit D supplementation can be an important public health action.

Key words: Oldest old, aged, 80 and over, vitamin D, depression.



The accelerated aging of Brazil has one of its epidemiologic consequences: the increased number of elderly with chronic diseases and incapacities that generate dependency (1). Late-life depression (LLD) affects from 10% to 22% of the growing geriatric population living in the community (2,3), it´s a risk factor for all-cause mortality in the elderly (4) and adults 85 and older appear to be more vulnerable to depression than other age groups (5). Wu et al. (6) demonstrates that the age-related growth of depressive symptoms occurs wholly in the context of medical comorbidity and does not have an independent effect. Weyerer et al. (7) found that the incidence of depression symptoms, measured using the GDS-15 Geriatric Depression Scale, increases significantly with age in non-demented primary care attenders aged 75 years and older. The presence of depressive symptoms as a risk factor for disability occurs in both genders (8) and it is associated with development of cognitive decline in older patients (9).
Nowadays, the relation between hypovitaminosis D and depression has been reported and it is estimated that 1 billion people worldwide have vitamin D deficiency or insufficiency (10). Hoogendijk et al. (11), in a cohort study, found that the lower levels of vitamin D were associated with higher intensity of depression. Milaneschi et al. (12), in 2010, also in a cohort study (InCHIANTI study), evaluated elderly of ages 65 and up and observed that hypovitaminosis D was a risk factor for the development of depressive symptoms in elderly. On the other hand, Toffanello et al. (13), in a prospectively studied population (Pro.V.A. study), showed that there was no direct effect between vitamin D deficiency and the onset of late-life depressive symptoms.
The oldest old people are not included or are under-represented in most of the studies. Because of that, we want to know if there is association between depressive symptoms and vitamin D in elderly aged 80 and over who are physically more active and independent.



Studied population

The analyzed data is part of a cohort study about free-living independent elderly aged 80 and over. The elderly have been following in the Geriatric Division from Federal University of São Paulo. We didn´t include oldest old people with dementia, cancer, acute disease, dialytic therapy, chemotherapy or radiotherapy.
The studied population included 182 oldest old people evaluated from the period of January 2010 to January 2012. The experimental protocols were approved by the appropriate institutional review committee and meet the guidelines of their responsible governmental agency. Informed consent was obtained from all individual participants included in the study (Federal University of São Paulo Ethical Committee approval number 1532/09).

Clinical assessment

The collected data were sex, age, ethnicity, precedence, smoking history (current, previous or more than one year without smoking, never smoked), alcohol history (drinking any amount of alcohol in the last 10 years), health perception (excellent, good, regular or bad), chronic pain (presence of pain for more than 3 months), and any exposition to sunlight. The neuropsychological evaluation was made by the Mini–mental state examination (MMSE) developed by Folstein and validated in Brazil by Brucki et al. (14). The functionality was evaluated by the Instrumental activities of their daily living (IADL) (15). The nutritional evaluation was made by the means of the Body mass index (BMI) (16), abdominal circumference (AC – we considered as a high AC value in elderly ≥ 102cm in men and ≥ 88cm in women), hip circumference (HC) and waist-to-hip ratio (WHR=CA/HC; WHR > 0,99cm2 in men or > 0,97 in women is associated with an increased cardiovascular risk) (17).
The approach of the depressive symptoms was done by the Geriatric Depression Scale (GDS) in its reduced 15 item version. Paradela et al. (18) validated the Portuguese version of the GDS to track depressive symptoms in ambulatory elderly, with a cut mark at 5/6 showing sensibility of 81% and specificity of 71%.

Biochemical analysis

The biochemical analysis of creatinine, fasting glycemia and serum hemoglobin was measured on a fasting blood specimens (collected after 10-hour fast). 25(OH)VitD analyses was done in serum sample refrigerated and protected from solar exposition. We used the DiaSorin LIAISON® 25(OH)VitD, which one is based on chemiluminescence technology (CLIA). We considered deficiency serum level of 25(OH)VitD <10ng/mL, insufficiency between 10 and 30ng/mL and sufficiency >30ng/mL (10).

Statistical analysis

For data processing we used “Statistical Package for the Social Sciences (SPSS) for Windows” (version 13). A measure of central tendency was represented by median and interquartile amplitude (IA) when appropriate. We used the bootstrapping method for assigning confidence intervals from the proportion and median. Levene’s test was used to assess the equality of variances for a variable calculated for two or more groups. We also used t Student´s test to determine if two sets of data were significantly different from each other, and the non-parametric tests Mann-Whitney (U) and Kruskal-Wallis (KW). When the Kruskal-Wallis (KW) test leads to significant results, Mann-Whitney (U)´s test was used with Bonferroni-corrected significance level. Chi-square test (X2) was used considering the recommendations of Cochran and the Fisher’s exact test when these recommendations were violated.



We studied independent oldest old people, with a IADL median 26,0 (IA 5,0) for men and 24,0 (IA 5,0) for women (p=0,187). Most of them were women and 82% of oldest old women never smoked (Table 1). The women had more depressive symptoms than men. On the other hand, oldest old men had a better performance on MMSE, with a schooling median 4,0 (IA= 5,5) for men and 3,0 (IA 3,3) for women (p=0,09). 83,7% of men and 88,7% of women had insufficiency or deficiency blood levels of 25(OH)Vit D although 52,2% declared sunlight exposition.
We also observed that 66,7% of men and 49,2% of women had excellent or good health perception (p=0,154). 77,6% of men and 62,4% of women did not have chronic pain (p=0,076) and 36,2% of men and 64,6% of women had abdominal circumference increased (X2=11,280; gl=1, p=0,001). There were no differences between serum levels of fasting glycemia of men compared to women (median 87,0 +/- 16,0 and 88,0 +/- 17,0 respectively). However, the men hemoglobin (average 13,7 +/- 1,6) was greater than for women (average 13,2 +/- 1,5; p=0.005).
According to blood level of 25(OH)Vit D (Table 2) we found difference between GDS score comparing the groups: “deficiency” (U=144,50; z=-3,126; p=0,002) and “insufficiency” groups (U=975,50; z=-2,793; p=0.005) are different than “sufficiency” group; but there was no difference between “deficiency” and “insufficiency” groups (U=1460,00; z=-1.263; p=0,206).

Table 1 Characterization of elderly aged 80 years and over according to gender

Table 1
Characterization of elderly aged 80 years and over according to gender

IA: interquartile amplitude; CI: confidence interval; SD: standard deviation; MMSE: Mini–mental state examination; GDS: Geriatric depression scale; BMI: Body mass index; Missing values a=9; *U=3116,50, z=-0,452; **U=2399,00, z=-2,678; ***U=2444, z=-2,605; ****U=2835,50, z=-0,428; #X2=1,505, gl=2; &Fisher’s exact test.



Table 2 Characterization of elderly aged 80 years and over according to levels of 25(OH)Vit D

Table 2
Characterization of elderly aged 80 years and over according to levels of 25(OH)Vit D

IA: interquartile amplitude;  CI: confidence interval; SD: standard deviation; MMSE: Mini–mental state examination; GDS: Geriatric depression scale; BMI: Body mass index; WHR= waist-to-hip ratio ; Clearance of creatinine: estimated clearance of creatinine. Missing values a= 9; b= 8; c=8; d=13; e=2. *KW=5,503, gl=2; **KW=1,539, gl=2; ***KW=10,743, gl=2; ****KW=1,954, gl=2; *****KW=9,103, gl=2; ******KW=2,485, gl=2; #X2=1,505, gl=2; ##X2=4,48, gl=2; ###X2=4,249, gl=2; & Fisher’s exact test.



In our cross-sectional study, we observed that there was association between worst GDS scores with < 30ng/mL of 25(OH)Vit D in oldest old people. It´s already known that depressive symptoms are associated with clinical 25(OH)Vit D deficiency (levels <10ng/mL) (19) in elderly 65 years of age. This was confirmed in a systematic review and meta-analysis of epidemiological studies: depression risk was found to be inversely associated with serum 25(OH)Vit D in both cross-sectional and cohort studies (20). But it seems that in free-living independent oldest old people the goal of 25(OH)Vit D levels can be higher to avoid depressive symptoms, levels under 30ng/mL can be inadequate. We have to consider that there is a decline of 25(OH)Vit D levels with age and also a gender difference (21) that is going to increase the risk of depression in oldest old age and can compromise functionality.
Low blood levels of 25(OH)Vit D can be related with the inflammatory status observed in depressed patients, because in these conditions, auto-reactive T cells against tissues and synthesis of the interleukins and the pro-inflammatory cytokines (IL-12, interferon gama) are stimulated by the immunologic system (22). Synthesis and metabolism of serotonin (5-hydroxytryptamine) is influenced by cytokine signaling pathways (23). In physiologic conditions, indoleamine 2,3-dioxigenase (IDO) compete by tryptophan hydroxylase (TH) in tryptophan metabolism. The activation of IDO metabolizes the tryptophan in kynurenine and in the end quinolinic acid. It decreases brain tryptophan and the serotonin levels.
The functional reserve decline with age and also the capability to the oldest old to maintain a health life style and independency. It´s interesting to note that these oldest old people are independent, free-living individuals and even so had 25(OH)Vit D levels under 30ng/mL. This was found for others researchers in elderly above 60 years of age (24, 25) despite their high sun exposure during the summer months and regarding the nutritional status (26).
It´s suggested that 25(OH)Vit D supplementation is indicated as a complement of depression treatment (27). Zanetidou et al. (28) demonstrated that administering 25(OH)Vit D to patients 65 years or older as an adjunct to antidepressant therapy was associated with a significant improvement in the depressive symptomatology. Considering that the costs are low and side effects are not common, 25(OH)Vit D supplementation is very cost-effective and can be a good choice to prevent depressive symptoms. This can be an important public health action to avoid depressive humor in oldest old people (29). We already know that to prevent fractures the goal is > 30ng/mL of 25(OH)Vit D (30) and it seems that, in oldest old people these levels are also recommended to avoid depressive symptoms. It´s important to establish if to avoid depressive symptoms in oldest old the goal is also > 30ng/mL of 25(OH)Vit D.
Our study has limitations: selection was by convenience and the GDS is a screening instrument and detect depression symptoms and not the diagnosis of depression.  It´s also important to note that 56,9% of the sample had an increased abdominal circumference that can be related with low levels of 25(OH)Vit D and also with a more inflammatory condition.
We conclude that the goal of 25(OH)Vit D levels can be higher to avoid depressive symptoms in free-living independent oldest old people and levels under 30ng/mL can be inadequate. Considering that the costs are low and side effects are not common, 25(OH)Vit D supplementation can be an important public health action.


Funding: This study was funded by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo – São Paulo Research Funding Foundation) – grant number 2011/12753-8. The sponsors had no role in the design and conduct of the study; in the collection, analysis, and interpretation of data; in the preparation of the manuscript; or in the review or approval of the manuscript.

Acknowledgements: We acknowledge and thank Ana Beatriz Galhardi Di Tommaso, Renato Laks, Paulo Mateus Costa Affonso, and all doctors who contributed in data collection.

Conflict of interest: Márcio Tomita da Rocha Lima, Osvladir Custódio, Patricia Ferreira do Prado Moreira, Lara Miguel Quirino Araujo, Clineu de Mello Almada Filho and Maysa Seabra Cendoroglo have no conflicts of interest to declare.

Ethical standards: This experiment complies with the current laws of the country in which they were performed.



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Products/3235.0~2012~Main+Features~Main+Features?OpenDocument. Accessed 9 August 2014


P.S. Tsindos, C. Itsiopoulos, A. Kouris-Blazos

Department of Rehabilitation, Nutrition and Sport, La Trobe University, Australia

Corresponding Author: P. Spero Tsindos, Department of Rehabilitation, Nutrition and Sport, La Trobe University, Bundoora, Victoria, 3086 Australia, Phone: +61 3 9244 6087, Fax: +61 3 5227 8130, Email: spero.tsindos@deakin.edu.au


Objective: This study examines a possible relationship between plain water consumption, mild cognitive impairment, depression and constipation in a cohort of Greek-born Australians aged 65 and over. Design: A cross-sectional study using a semi-quantitative food frequency and lifestyle questionnaire. Participants: We recruited 150 elderly Greek migrant volunteers who were born on Greek Mediterranean islands and collected detailed diet and lifestyle data using an established protocol used in the Mediterranean Islands Study (MEDIS) in Greece. Measurements: Water intake from plain water, beverages and foods was assessed using a validated food frequency questionnaire. Depression and memory were assessed using the validated Geriatric Depression Scale – Short version. Results: Mean water intake from all sources for all participants was 2871 mL (p = 0.010), water derived from foods was 1048mL (p = 0.014), beverage intake other than plain water was 876mL (p < 0.001) and plain water consumption was low with a mean of 947mL (p = 0.001) per day. Those who reported as not depressed (GDS < 6) consumed 100 mL less total water from food and beverages than those who scored 6 or above and those who self-reported no constipation consumed nearly 300mL more water in foods than those who self-reported being constipated. Conclusion: Results suggest that habitual low consumption of plain water (< 1000mL/day) was not associated with constipation or self-reported mental-emotional disorders in this group. Water consumption from food was significantly higher in those with no constipation suggesting that consumption of water in food may be a significant factor in ensuring adequate water needs. Clinicians should consider water intake in food when assessing patient water intake.

Key words: Drinking, hydration, mild cognitive impairment, depression, constipation.



For a number of years there has been a strong public movement arguing the need to drink two litres or eight glasses of plain water a day (1). Despite a lack of any tangible evidence, this argument has become commonplace with claims that failing to provide an abundance of water, puts the body in jeopardy (2, 3).

The view that water deprivation can lead to significant physical or mental dysfunction has not been well-researched. There is some evidence that poor consumption of plain water can cause an increased risk of cardiovascular disease, particularly in older men (4-7). As humans age, thirst diminishes and replenishment slows. (8) For older persons, this dysfunction can lead to a range of both physical and mental problems (6).

Researchers have noted that both depression and mild cognitive impairment (MCI) can occur concomitantly in older persons and that an inadequate water intake may initiate or exacerbate both conditions (9-12). MCI in particular can become evident and worsen when total body water (TBW) drops by 1-3% (13-15). Szinnai, et al, (2005), investigated this relationship between water loss and MCI, with inconclusive results, suggesting in their conclusion that the withdrawal of caffeine may have attributed to some of the outcome (16). Other researchers have also argued that the evidence for this relationship is not definitive (12, 17). As a consequence, the evidence in support of a direct relationship between depression, MCI decline and hydration is not clear suggesting further research is required in this area. Further, there has been investigation into the relationship between diet and MCI, suggesting that a poor diet may have an influence in the progression of the condition (18).

Although there can be significant physiological problems associated with water deprivation in older persons, the research has not been forthcoming. When water intake is less than water output (hypohydration), there are physiological consequences. In a mild, chronic hypohydrated state, problems can include constipation and urinary tract infections (19, 20). Constipation in particular, is considered a common consequence of low water intake (19). It is estimated 15-20% of community-living older persons suffer from constipation and a higher percentage in residential care (21). The significance of this disorder can vary depending a variety of factors, such as low fibre and on the extent of hypohydration.

Lastly, the determination of water requirement and whether it is adequate has been shown to be a difficult value to estimate. Although there has been a great deal of research into the problem of hypohydration, it has focussed on infants and children, those engaged in physical activities such as sport or within the military (22, 23). In relation to any effect on mental states, the focus has been on ambient temperature and water loss through sweating (24, 25). When considering effects of water intake on older persons, there has been little research conducted in this area when there is little physical activity and minimal exposure to high temperatures. (26) What has been investigated in this area is the effect of dehydration on older persons who have a diminished thirst response, a condition associated with ageing (27).

If a criterion for healthy living in older persons is consuming two litres of plain water a day, we would expect to see at least some of the above problems when less than two litres of plain water per day is consumed. Older Greek migrants in Australia have been extensively studied because of their mortality advantage over the Australian-born (28). Their desirable food habits have been linked to their lower mortality and CVD and diabetes risk (29). However, studies failed to investigate the role of water intake of older Greek-Australians with respect to health. Exploring the water intake of long-lived older Greek-Australians may help further our understanding of water consumption and its effect on health.


Using the dietary guidelines recommended by the Supreme Scientific Health Council of Greece (30), as a guide to estimating water requirements from a healthy Mediterranean diet model (from beverages and foods), we investigated whether failure to drink two litres of plain water a day may be associated with health implications for older persons. We therefore investigated the food and fluid intake of 139 older Greek Australians aged over 65 (as part of the epidemiological study MEDIS [MEDiterranean Islands Study]) to determine,

1. the relationship between plain water intake and mood, memory and bowel habits (plain water, in this instance is considered tap water, bottled water or filtered water with no flavourings).

2. methods by which water requirements can be estimated non-invasively.

The MEDIS-Australia study was conducted to compare health and lifestyle data of older Greek immigrants who were born on Greek Islands with that of the MEDIS study of the Harokopio University of Athens, Greece who investigated the health status of elderly Greek Islanders currently living on Greek islands. (31) The MEDIS-Australia study consisted of an extensive semi-quantitative food frequency questionnaire (MEDIS-FFQ) and a detailed semi-structured lifestyle questionnaire (MEDIS-LQ). This study was reviewed and approved by the University Human Research and Ethics Committee in accordance with the National Health and Medical Research Council of Australia’s National Statement on Ethical Conduct in Human Research (2007) .The study gathered information from a total of 179 participants, of which 139 completed the questionnaires fully and provided medical records. Although the MEDIS-Australia data was originally designed to evaluate cardiovascular risk factors and establish a Mediterranean diet pattern score, we extracted relevant data to examine participant’s self-reported food and water intake, depressive state (including the subjective perception of memory – a measure of cognition) and whether they suffered from constipation in association with hypohydration. Table 1, shows the overall demographics of the participants. The majority of participants were of Cretan or Cypriot origin (52 and 74 respectively) with the remainder from other islands, and one from the Greek mainland. There were more females than males with a mean age of 74 years. Compliance was high to a Mediterranean diet pattern, with only one participant scoring below 26 within a scale from zero to 55. It has been shown that a score of 26 or more shows a strong adherence to a Mediterranean diet pattern (32).

Table 1 Origin and Anthropometric Data of Participants of the MEDIS-Australia Study (n = 141)

*Other islands includes Corfu, Ithaki, Mitilini, Samos and one from mainland Greece

A number of factors can make interpretation and determination of the diagnosis of depression difficult in older adults. Factors can include a concomitant illness, an absence of an obvious depressed mood, and social isolation (33). Evaluation of the mental health of the participants of the MEDIS-Australia Study was through the administration of the Geriatric Depression Scale – Short version (GDS-S). The 30-item Geriatric Depression Scale has been used widely, and this short version (15 items) had been evaluated as a useful, quick alternative to the full version, which was thought to take too long to complete (34). The GDS-S also includes a question relating to memory. In the GDS-S a score of zero to five is considered normal, six to nine suggests mild depression, and a score of ten or more suggests moderate to severe depression. Question 10 of the GDS-S states, “Do you feel you have more problems with your memory than most?” Analysis of this question along with concomitant GDS-S scores and Water intake, were examined.

In the Lifestyle Questionnaire of the MEDIS-Australia Study, eight questions were asked regarding bowel habits, starting with whether the participant was constipated, if so, in what manner was the problem addressed and whether there was a familial history. Constipation was self-reported rather than diagnosed.

Additionally, an examination of water requirement was undertaken to determine what quantity of water would be required to ensure individuals were receiving enough to maintain adequate hydration. Initial calculations regarding water content of foods and beverages were undertaken using the NUTTAB 2013 and AUSNUT 2007 databases, processed through FoodWorks 7.0 Professional software. All statistical analysis was undertaken using IBM SPSS Statistics version 22. Analyses were adjusted for covariates, such as specific diuretics and medications that induced a diuretic action using multivariate linear regression and non-parametric analysis.

Normal water loss can occur through five different mechanisms, sweat, urine and faeces, insensible water loss and normal respiration (26). This water loss is influenced by activity, metabolic rate, ambient temperature and humidity. To reasonably estimate water loss and hence water requirements in individuals, these factors need consideration. Firstly, MEDIS-Australia participant’s activity was minimal with most participants reporting particularly sedentary lifestyles. Consequently, no adjustment was required for activity. Secondly, according to data received from the Bureau of Meteorology Australia, the mean temperature across the study timeframe from March 2012 through to March 2014 was 16.1⁰C (±0.22) with a mean humidity of 64% (± 3.4) on those days and months when the interviews took place (35). Thirdly, it is assumed that the metabolic production of water is offset by respiratory water loss.

Given the above factors a novel approach may be considered for estimating water requirements, for MEDIS-Australia participants using a formula. Three formulas were proposed by Bossingham, et al, (36), which are outlined in Table 2, along with their primary reference sources.

Table 2 Formulas for Water Intake Estimates (WIE), independent of age


WIE1 relies on energy intake while WIE2 and WIE3 rely on body weight. WIE1 has been used often since 1945 when first published in the Recommended Dietary Allowances. The recommendation is applied as a footnote to the Recommended Dietary Allowances table and there is no further reference for this recommendation (37). WIE2 also does not supply any further reference, but states, “The fluid requirements for older adults is usually calculated as 30 mL/kg body weight with a minimum requirement of 1500 mL/day”. Skipper, the reference for WIE3, references the Manual of Pediatric Nutrition, by Kerner, (1983).

Table 3 indicates that both skewness and kurtosis suggest that WIE2 or WIE3 would be suitable as a method of estimation. Skewness and kurtosis approached zero at 0.17 and -0.255 respectively, compared to WIE1, which had a skewness of 1.399 and kurtosis of 4.91. As the Confidence Interval for WIE2 had a greater range than that of WIE3, we chose WIE3 as our formula for estimating water intake.

Table 3 Descriptive statistics of Water Intake Estimates (WIE) (n=141)*

*Water intake estimate Mean and Median in mL.

Each participant completed an extensive semi-quantitative food frequency questionnaire (MEDIS-FFQ) comprising food frequency and portion size questions for 112 different foods. Portion sizes were determined using images developed for the Australian Guide to Healthy Eating (38), for estimation of food portion sizes. Each food was analysed for water content using the Australian Food, Supplement and Nutrient Database and FoodWorks Professional 7.0. Lifestyle profiles were developed using the MEDIS-LQ, which incorporated the GDS-S.


All participants (n = 139) completed the GDS-S. Due to the small sample size when dividing by gender, an initial analysis of the data was undertaken. Using a Mann-Whitney U Test, the distribution of Water and the GDS-S range were the same for both males and females. Therefore all participants in the analysis were included.

Further, analysis of the ranges within the GDS-S and the relative consumption of water provides the results as shown in Table 4. The mean total volume of water from all sources consumed by all participants was 2871 mL (SEM 72.9). The mean total intake of all water for those considered within the normal range of the GDS-S was 2800 mL (SEM 136.5). Mean total water intake was not statistically different in mild and moderate to severe depression. When we examine the volume of plain water consumed, for those who scored in the moderate to severe depression range we found that there was a higher mean volume intake of plain water (1010 mL (SEM 136.3)) compared to 947 mL (SEM 42.8) per day for those who scored as not depressed.

Table 4 Mean and standard error (SEM) for water intake measured against GDS-S score and responses to the question, “Do you feel you have more problems with your memory than others?” (n=139)

*Water calculated from all sources, both food and beverages; ǂ Water found only in foods not considered beverages consisting of 45.2% vegetables, 20.5% fruit, 19.6% dairy products (other than milk), 6.3% of poultry, eggs, red meat and sweets, 5.6% cereals and 2.8% fish; §A beverage is defined as, “any kind of drink other than plain water” consisting of 26% milk, 15% sweet drinks, 11% alcoholic drinks, and 48% hot drinks, such as teas and coffees; ǁPlain water includes mineral or carbonated water, bottled water with no additives and tap water


When examining the data in Table 4, it is apparent that the total water consumed in all cases, met or exceeded the volume of water estimated as adequate (in this instance, 2,360 mL as shown in Table 3). As can be seen, the highest percentage of beverage intake was from teas and coffees at 48%. Despite their caffeine content, it has been shown that habitual consumption of caffeinated beverages, such as teas and coffees diminishes the diuretic action of caffeine (39). As participants indicated they drank caffeinated beverages on a daily basis, the diuretic action of the caffeine would be minimal. A Mann-Whitney U Test indicated there was no significant difference between the levels of depression and water intake from all sources. The total water intake maintains a relative stability as the GDS-S range increases even though the volume of plain water rises. Despite this rise in plain water intake, it still did not meet or exceed the generally recommended consumption of two litres of water a day. This further suggests that the water consumption is not associated with the participant’s state of depression.

Table 4 also shows the relationship between the scores from the GDS-S and the relevant score for Question 10 of the GDS-S, “Do you feel you have more problems with your memory than others?”

When considering the volume of water consumed as noted in Table 4, the volume of total water is consistent across all depressive states. We can argue that in this population sample, different volumes of water intake is not associated with memory problems. The relationships between the score for Question 10, and the overall depression scale scores show that those who answered “No” to the question consumed less water overall and less plain water in particular, than their counterparts who answered “Yes” to Question 10. The only exception is the plain water volume for those who scored between 6 and 9 on the GDS-S. All participants who answered “No” to Question 10, showed a total water intake of 2805 mL (SEM 83.8) as compared to those who answered “Yes” with a total water intake of 3140 mL (SEM 132.8). This suggests that consuming less than the recommended two litres of plain water per day is not associated with a negative impact on the mood or mental capabilities of these participants. Although this is the case, it needs to be understood that these responses cannot be seen as definitive without objective verification

One of the more common physical problems attributed to a low water intake is constipation (19). We examined the question posed in the MEDIS LQ asking the participants whether they were constipated or not and how this problem was treated. The results of the questions are shown in Table 5, with relevant water volumes.


Table 5 Mean and Standard Error (SEM) of Fluid Intakes from Various Sources and Constipation (n = 119)

*1 = up to 1-2 times per week; 2 = 3-5 times per week, 3 = more than 1 time per day

Of the total number of participants, 123 answered the questions relating to constipation. Four participants did not provide FFQ data, which left a cohort of 119 for analysis.

Table 5 shows the following results, 28 participants, identified as constipated and consumed less total water (2751 mL (SEM 161.8)) than the 91 participants who did not identify as constipated (2967 mL (SEM 92.7)). Although, those who admitted they were constipated consumed a mean of 1050 mL (SEM 72.3) of water in the food they ate compared to 1097 mL (SEM 47.4) in the foods of those who claimed not to be constipated, the group who reported they were not constipated consumed more beverages than the group who claimed to be constipated (approximately 200 mL more). Overall when the totals of food water and beverage water are combined, those who claimed to be constipated consumed a combined mean of water in these two categories of 245 mL less than those who claimed not to be constipated. Both groups consumed approximately the same volume of plain water, slightly less for those who claimed not to be constipated (991 mL (SEM 77.3) compared to 962 mL (SEM 58.6)). For the question, “How frequently do you go to the toilet?” the group with the highest frequency of bowel movements (Group 3) consumed the highest total water volume (3128 mL (SEM 132.4)). However, this group did not consume the highest volume of plain water, which was attributed to Group 2 (1048 mL (SEM 116.1)). The lowest mean of total water intake (2296 mL (SEM 222.2)) was attributed to Group 1, which has the least frequent bowel motions. Additionally, Group 3 had a higher volume of water from food than Group 1 (a difference of 223 mL). Further, when testing for model effects, the level of activity and the taking of diuretic medications did not influence the outcome in any significant way. Another important observation is that the difference in water intake between those who claimed to be constipated and those in Group 3 was highest in the water in foods category. As fruits and vegetables were highly represented in this category, consuming water through foods would also add fibre and nutrients which could in part influence bowel habits. All groups who self-reported bowel habits fell short of the expected two litres of plain water a day suggesting that normal bowel function within this group of older adults was not compromised by a self-reported volume of plain water significantly below the suggested two litres of water per day.

Discussion and conclusion

The expectation that an individual needs to consume two litres of plain water in order to maintain good health has been brought into question commencing with the article by Valtin (2002). The evidence for the consumption of two litres of plain water a day has not been forthcoming and on the contrary, a growing body of evidence disputes this claim (1, 3). The recommended quantity may be unnecessary, and unrealistic.

Depression is defined as a serious medical illness characterised by deep feelings of sadness and loss of interest or pleasure in activities (33). Mild cognitive impairment (MCI) is, “…a syndrome defined as cognitive decline greater than that expected for an individual’s age and education level but that does not interfere notably

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with activities of daily life” (9). Identification of MCI is either from the individual concerned or a knowledgeable informant. In this study, we were able to examine self-reported memory difficulties using the GDS-S questionnaire.

The possible association examined between that of depression, memory problems and water intake suggested that those who scored high in the GDS-S consumed less water from foods overall than all other categories. Also, this group of participants consumed more plain water than the other groups. People that reported not being depressed consumed the highest volume of plain water than other groups when answering “yes” to Question 10. This group of participants had a similar consumption of water in foods with the group who tested as moderately depressed, but was lowest for beverages other than water.

There appeared to be an apparent association between the ingestion of water, both as part of foods and beverages and as plain water, with constipation, namely those reporting higher water intake (through food and plain water) were less likely to be constipated. Although this association was evident, the volume of plain water per day was significantly lower than two litres. As these participants scored moderate to high with a Mediterranean diet pattern score and the Mediterranean diet is known to by high in vegetables and fruit, a high volume of water intake from these foods will likely have a concomitant high quantity of fibre. One limitation of this study was the need to compare fibre intake alongside that of water intake. This small cross-sectional study suggests that this group of older Greek-Australian migrants do not appear to be affected by lower than recommended water intakes.

Our study results support the view that community-living older persons have a low likelihood of hypohydration based on adequate water intake from foods. Further there may be other factors for consideration in this regard, such as cultural and communal behaviours that may influence food and water consumption.

The adequate intake of water has been an elusive standard and can be difficult to quantify due to factors such as environment and activity level. In our investigation older Greek-Australians consumed adequate water to meet their estimated water requirement, suggesting that the formula we used to calculate water needs may be useful. However, there are a number of confounders that should be considered. The ambient temperature and humidity are relevant in the estimation of water requirement using a predictive formula, particularly when estimating for physical activity. In this study participants were older and led very sedentary lifestyles.

As our investigation has indicated, a high intake of fresh fruits and vegetables will contribute to the overall water intake. The recommended five vegetables and three fruit portions a day are a suitable target to assist in meeting fluid requirements. This will not only provide water in a plant medium, but also fibre and other nutrients favourable for optimum health. The Mediterranean diet pattern is high in plant-based foods with a high water content. This pattern of eating ensures that community-living older persons may not be at risk of an inadequate water intake when following the Mediterranean diet pattern.

This investigation derived data from the MEDIS-Australia study, which was not specifically designed to capture water intake. The nature of this cross-sectional study cannot show a causal relationship between the consumption of water and health outcomes. Also, the intake of other nutrients known to influence bowel habits, such as fibre were not specifically measured and given that the participants ate a diet quite high in fruit and vegetables, this limitation may be significant.

As there appears an association between water intakes, both in the form of foods as well as plain water, more research in this area may shed further light on the possible underlying relationship between health and water consumption. The only effective way to determine this relationship would be with a longitudinal study, possibly by follow-up of the existing MEDIS-Australia participants. The information derived from the MEDIS-Australia study was comprehensive and provided insight into the eating and lifestyle behaviours of a group of immigrants to Australia. Research into other ethnic groups and already established Anglo-Celtic Australians would further provide insight into how significantly eating and lifestyle behaviours determine water intake and subsequently health.

Ethical Standards: This study was reviewed and approved by the La Trobe University Human Research and Ethics Committee and assigned the Approval number: HEC11-045 in accordance with the National Health and Medical Research Council of Australia’s National Statement on Ethical Conduct in Human Research (2007)


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