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ALTERATIONS OF THE ORAL MICROBIOTA IN MILD ALZHEIMER’S DISEASE AND THE APPROPRIATE APPLICATION OF CHLORHEXIDINE GLUCONATE

Huizhen Cao, Jiangming Zhong, Lili Chen

J Aging Res & Lifestyle 2025;14

OBJECTIVE: This study investigated the effect of 0.2 % chlorhexidine gluconate on oral microbiota dysbiosis in Alzheimer’s disease (AD) and explored potential links between oral microbiota and cognition, offering new insights into its role in AD treatment. STUDY DESIGN: We assessed the impact of 0.2 % chlorhexidine gluconate on the oral microbiota of patients with AD. One hundred patients were divided into two groups based on oral health score (using a cut-off of 8). Subgingival plaque samples were analyzed using 16S rRNA sequencing; no significant differences in bacterial composition were observed between groups at baseline. RESULTS: Poor oral health correlated with higher oral health scores (P = 0.000), fewer teeth (P = 0.002), lower cognitive levels (P = 0.048), and a higher proportion of patients with diabetes (P = 0.032). After 24 weeks of treatment with 0.2 % chlorhexidine gluconate in a randomized controlled trial, subgingival plaques from 66 patients showed changes in Porphyromonas, Filifactor, Desulfobulbus, Anaeroglobus, Pyramidobacter, Mycoplasma, Dialister, Fretibacterium, and Tannerella (P < 0.05). Treponema and Porphyromonas gingivalis were identified as potential interventional targets. CONCLUSIONS: Chlorhexidine gluconate effectively alters oral flora, reducing harmful bacteria. Targeting specific microbiota disturbances may offer a promising strategy to delay AD onset or slow its progression.

CITATION:
Huizhen Cao ; Jiangming Zhong ; Lili Chen (2025): Alterations of the oral microbiota in mild Alzheimer’s disease and the appropriate application of chlorhexidine gluconate. The Journal of Aging and Lifestyle (JARLife). https://doi.org/10.1016/j.jarlif.2025.100024

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