STIMULATORY EFFECT OF ACUTE SINGLE DOSE OF DRIED WHOLE COFFEE CHERRY POWDER ON NRF2 ACTIVITY IN FRESHLY ISOLATED BLOOD CELLS. A SINGLE-BLIND, PLACEBO CONTROLLED CROSS-OVER PILOT CLINICAL STUDY
T. Reyes-Izquierdo, B. Nemzer, R. Argumedo, M. Cervantes, Z. Pietrzkowski
J Aging Res Clin Practice 2016;5(3):120-127
Abstract: Background: NF-E2-related factor 2 (Nrf2) is a transcription factor that participates in the regulation of antioxidant expression during increased oxidant stress. Several phytochemicals and food products have shown to trigger Nrf2 activity. In this pilot placebo-controlled study the effects of a single dose of dried whole coffee cherry powder (“WCCP”) on Nrf2 levels were tested. Objectives: To characterize a blend of WCCP and evaluate the potential effects on mTOR and Nrf2 in healthy subjects. Design: In this cross over study, subjects were given placebo or a single dose of 1000mg WCCP on day 1 and 2. Blood was collected for four time points. Participants served as their own controls. Setting: After supplementation, blood samples were processed for mTOR and Nrf2 analysis. Blood ATP, glucose and lactate were also measured. Participants: Ten healthy subjects, ages ranging from 22 to 35 years and BMI ranging from 24.1 to 30 kg/m² were selected to participate. Results: One 1000 mg dose of WCCP resulted in an average 44% increase of NRf2 levels 180 minutes after ingestion (p=0.03 ). Phosphorylated mTOR (Ser 2448) was reduced at 180 minutes after supplement; when compared to placebo. Correlation (“Corr”) analyses revealed that increases in Nrf2 appear to be associated with mTOR reduction. Blood glucose and extracellular ATP levels were not changed. Conclusions: WCCP increased Nrf2 3 hours after ingestion. Additional testing is required to verify the potency of WCCP on Nrf2, as well as any potential correlation between mTOR (S2448) reduction and increased levels of Nrf2 after supplementation.
T. Reyes-Izquierdo ; B. Nemzer ; R. Argumedo ; M. Cervantes ; Z. Pietrzkowski (2016): Stimulatory effect of acute single dose of dried whole coffee cherry powder on Nrf2 activity in freshly isolated blood cells. A single-blind, placebo controlled cross-over pilot clinical study. The Journal of Aging Research and Clinical Practice (JARCP). http://dx.doi.org/10.14283/jarcp.2016.109