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DOES LONG-TERM ADMINISTRATION OF BIOLOGICALLY ACTIVE SUBSTANCES-ENRICHED DIET INFLUENCE GENERAL HEALTH STATUS PARAMETERS IN RATS?

H. Oszkiel, J. Wilczak, A. Prostek, D. Kamola, M. Hulanicka, M. Jank

J Aging Res Clin Practice 2014;3(2):63-71

As the concept of diet supplementation with biologically active substances becomes more and more popular in humans, and manufacturers of the supplements encourage people to take multiple biologically active compounds simultaneously, we wanted to evaluate the influence of long term (14-months) use of biologically active substances-enriched diet (BASE–diet) on blood morphology, liver function, oxidative stress and antioxidant defence in rats. The experiment was conducted on 54 Sprague - Dawley rats divided into two experimental groups (fed with control or BASE-diet, both n=27). Control diet was a semi-synthetic diet formulated according to the nutritional requirements for laboratory animals (1). The BASE-diet was enriched with a mixture of polyphenolic compounds, β-carotene, probiotics, and n-3 and n-6 polyunsaturated fatty acids. None of the major blood parameters were influenced by the 14-months-long use of BASE-diet. The diet also did not influence the values of ALP, ALT, GGT and BIL in both the liver tissue and plasma after 3 and 14 months of feeding. However, a significant decrease of the TBARS was observed in the liver after 6 and 14 months application of the BASE-diet. Furthermore, GSH/GSSG ratio was increased, GSH-Px and GSSG-R activities were decreased, and a reduction of SOD activity was noted in rats fed BASE-diet, when compared to control animals. Our data clearly show that long-term use of diet enriched with different biologically active substances is completely safe, and may improve the antioxidant mechanisms in blood and liver.

CITATION:
H. Oszkiel ; J. Wilczak ; A. Prostek ; D. Kamola ; M. Hulanicka ; M. Jank (2014): DOES LONG-TERM ADMINISTRATION OF BIOLOGICALLY ACTIVE SUBSTANCES-ENRICHED DIET INFLUENCE GENERAL HEALTH STATUS PARAMETERS IN RATS?. The Journal of Aging Research and Clinical Practice (JARCP). http://dx.doi.org/10.14283/jarcp.2014.13

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