M.M. Essa1,2, N. Braidy3, W. Bridge4, S. Subash1,2, T. Manivasagam5, R.K. Vijayan1, S. Al-Adawi2,6, G.J. Guillemin7
1. Department of Food Science and Nutrition, College of Agriculture and Marine, Sciences, Sultan Qaboos University, Oman; 2. Ageing and Dementia Research Group, Sultan Qaboos Unviersity, Oman; 3. Centre for Healthy Ageing, School of Psychiatry, Faculty of Medicine, University of New South Wales, Sydney, Australia; 4 School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, Australia; 5. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Tamil Nadu, India; 6. Department of Behavioural Medicine, College of Medicine and Health Sciences, Sultan Qaboos University, Oman; 7. Neuropharmacology group, MND and Neurodegenerative diseases Research Centre, Macquarie University, NSW, Australia
Corresponding Author: G.J. Guillemin, Neuroinflammation group MND and Neurodegenerative diseases Research Group, Australian School of Advanced Medicine (ASAM), Macquarie University, NSW, 2109 Australia. Tel.: +61 02 9850 2727; fax: +61 02 9850 2701. E-mail address: email@example.com
This review examines evidence of plant-derived natural products and their constituents that have been shown to slow down or reverse the underlying neuronal degeneration observed in Parkinson’s disease (PD), with a focus on their effect on the modulation of dopaminergic neurotransission levels and motor function. During the last decade, there have been over 140 studies published that have investigated the anti-PD therapeutic potential of herbs, fruits, vegetables and spices, ornamental and parasitic plants, and fungi. Empirical evidence implicates phytochemicals may play a role in the prevention and mitigation of some of the intractable signs and symptoms of PD. The anti-PD effects exhibited by these natural products are considered to be due to their ability to modulate; reactive oxygen species production, neuroinflammation, dopamine production, excitotoxicity, metal homeostasis, mitochondrial function, and cellular signaling pathways, which are all disrupted in the PD brain. However, the precise neuroprotective mechanism of action of natural products for PD remains unclear. Research is necessary to further elucidate the mechanisms by which these compounds are efficacious in attenuating PD or controlling PD-related symptoms.
Key words: Polyphenols, Parkinson’s disease, oxidative stress, mitochondrial dysfunction, neurodegeneration.
Parkinson’s disease (PD) is the world’s second most common neurodegenerative disorder, which can significantly impair the quality of life, create dependency and trigger premature mortality of affected individuals (1). The prevalence rate of PD is 0.5-1% among people aged 65-69 years and 1-3% among those aged 80 and above (2). Clinical manifestations include bradykinesia, tremors, rigidity, and postural instability (3). Spectrums of non-motor symptoms are also common, including cognitive impairment, and emotional and behavioral dysfunction (4).
As yet, no definite etiological factors have been identified to contribute to the development and progression of PD, although many factors have been previously proposed (5). Pathologically, PD is characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta region of the brain (7, 8). Intracytoplasmic proteinaceous inclusions termed Lewy bodies (LBs) and dystrophic neurites (Lewy neurites) are present in surviving neurons of PD patients (8). Genetic factors such as mutations to the α-synuclein or the parkin gene, and environmental factors such as neurotoxic pollutants have also been proposed to contribute to the onset of PD (9-11).
The neurochemical events associated with the pathology of PD include increased levels of free radicals, oxidative stress, inflammation, mitochondrial dysfunction, and α-synuclein aggregation. Additionally, increased concentration of redox active metals such as iron and copper, reduced glutathione levels, and increased lipid peroxidation have also been reported in PD (12, 13). Currently available pharmacological interventions provide only limited symptomatic relief for patients with PD and have little efficacy in reversing the underlying neuropathological changes associated with the disease. Therefore there is a clinical need to identify therapeutic agents that can ameliorate, or slow down the deleterious processes associated with PD. One such paradigm is to explore the possible contribution of natural products that might interfere with PD pathology (14). Natural products have been increasing found to have specific molecular or pharmacological effects that are likely to contribute to the development of neuroprotective agents against PD (15).
Bioactive derivatives of plants such as flavonoids, stilbenoids and alkaloids possess potent anti-oxidative and anti-inflammatory properties that are of considerable interest for the treatment of PD. These naturally occurring phytochemicals can also promote mitochondrial function and serve as important cognitive enhancers (15). Moreover, these compounds act as inhibitors for α- synuclein aggregation, c-Ju N-terminal kinase (JNK) activation, and monoamine oxidase production, and are agonists for dopaminergic neurons (16, 17). Considering the socioeconomic burden and undesirable side effects of synthetic drugs, natural remedies are promising avenues in the treatment of PD.
However, the claims that many natural products available to the general population are therapeutically beneficial for PD are based solely on empirical or preliminary data. Therefore, the purposes of this review are to highlight available evidence on the role of specific bioactive molecules present in a wide range of natural flora, and to present information on the potential mechanism of action of these natural products, and their relationship to neuropathological events associated with PD.
Summary of the literature cited
There are over 140 studies published in the last 10 years associated with the beneficial effects of plant- derived natural products and PD. This review included electronic searches of the PubMed, Medline, Scopus, EMBASE, CINAHL, AMED, PsycInfo, CNKI, 7 Korean Medical Databases, J-East and Web of Science with the search terms as follows: “Parkinson’s disease therapy”, “natural products parkinson’s disease”, “phytochemicals”, “antioxidants” and “plant extracts” in various combinations. The “Dopamine and PD” section is provided since the etiology of PD is thought to be due to an imbalance in brain dopamine levels. This review presents a summary of literature on the relationship between dopamine (DA) levels and motor function in PD, and the beneficial effects of natural products which demonstrate potential anti-PD properties. This also review summarizes recent progress in determining the potential mechanism of action on PD.
Dopamine Levels and Parkinson’s Disease
DA is a neurotransmitter produced from the dietary amino acid tyrosine and plays significant roles in a variety of motor, cognitive, motivational, and neuroendocrine functions (18). The rate-limiting enzyme responsible for DA synthesis is tyrosine hydroxylase (TyrH), which catalyzes the hydroxylation of tyrosine to DA. The biosynthetic pathway leads to the production of a number of different catechol monoamines, such as epinephrine and norepinephrine. These products play significant roles in many brain functions, including attention, memory, and cognition. Therefore, deficits in catecholamine synthesis can lead to several deleterious processes, such as hypertension, depression, and dystonias (reviewed in 19). As TyrH is the slowest enzyme in this pathway, its mechanisms of regulation are of considerable interest to neuroscientisits.
The activity of TyrH is regulated by protein-protein interactions with other enzymes in the DA synthesis pathway or the tetrahydrobiopterin pathway , an important cofactor in several redox reactions and acts as a chaperone for the maintenance of normal neuronal oxidative status (19). TyrH is also regulated by protein which transfer DA into synaptic vesicles. Recent studies have shown that TyrH is localized in close proximity to effector proteins near neuronal vesicles and mitochondria, along with protein phosphatases, and aromatic amino acid decarboxylase (AADC), and the vesicular monoamine transporter-2 (VMAT). These vesicles contain the enzyme dopamine beta-hydroxylase, thus ensuring that DA is stored in secretory vesicles rather than freely located in the cytosol (19). TyrH activity is inhibited by reversible glutathionylation during chronic oxidative stress, and the effect may be attenuated by natural phytochemicals which exhibit potent antioxidant potential (20).
DA is unstable and cannot cross the blood brain barrier (BBB). It is formed in the brain by conversion of its precursor L-DOPA. In PD, L-DOPA (Levodopa) can be administered in conjunction with a DA agonist, or the decarboxylase inhibitor carbidopa, which increases plasma concentration of L-DOPA by blockage of peripheral degradation of L-DOPA to DA. This allows more L-DOPA to BBB (21). L-DOPA is naturally found in beans, especially Mucuna spp, which has been proven experimentally to enhance DA levels in the brain (22).
DA receptor agonists (e.g. bromocriptine, cabergoline, pergolide, rotigotine, apomorphine, ropinirole and pamipexole) are the main class of drugs used to treat PD symptoms. Parkinson’s symptoms occur in response to reduced levels of the chemical messenger DA, due to the progressive loss of neuronal cells in the brain that synthesize it. These drugs activate DA receptors by bypassing the presynaptic synthesis of DA. Like DA, these DA agonists serve as endogenous free-radical scavengers, regulating DA synthesis and ameliorating excitotoxicity by suppressing subthalamic nucleus overactivity and exerting anti-apoptotic effects (23-25). Additionally, DA receptors can regulate adenylyl cyclase activity and cAMP synthesis (26, 27), and are involved in modulating voltage dependent Ca2+ and K+ channels (28). Compared to levodopa, DA agonists provide modest symptomatic relief but are associated with higher incidence of adverse effects including hallucinations, edema, sudden sleep attacks, and impulse control disorders (ICD). Interestingly, motor fluctuations such as dyskinesias are less frequent following administration with DA agonists. Naturally occurring protoalkaloids and ergot alkaloids are phytochemicals that exert dopaminergic or DA receptor activity. For example, apomorphine derived from opium alkaloids is used as a DA agonist (29).
Monoamine inhibitor (MAOI) activity of plant derived alkaloids is another therapeutic target for PD. Monoamine oxidases (MAO A & MAO B) are flavin- containing enzymes in the central and peripheral nervous systems that catalyze the conversion of neurotransmitters to hydrogen peroxide (H2O2), aldehydes, and ammonia. In the PD brain, MAOI regulates the breakdown of DA to its metabolites (30, 31). Therefore, MAOIs can be used therapeutically to maintain optimum DA levels (32).
Herb Derived Anti-parkinsonian Compounds
The neuroprotective potential of natural products derived from herbs, fruits, vegetables, and spices against PD relies on the presence of flavonoids, steroidal lactones, ginsenosides, alkaloids, caffeine, stilbenoids, ginkgolides, bilobalides , xanthones, saponins, oligosaccharide esters, glycosides isoflavonoids, polymethoxyflavones, catechins, anthocyanins, S- Allylcysteine, lycopene, thymoquinone, sesaminoids,curcuminoids, zingerone, eugenol, and chrysotoxine. enoids, catechols and glycosides. Flavonoids, the major polyphenol group, consist of aromatic rings possessing a phenolic hydroxyl group and a 3-OH group offering potent antioxidant and iron chelating properties (33-35). Based on their alkylation, glycation, and hydroxylation patterns, flavonoids are classified into flavones, flavanones, flavanonols, flavanols, anthocyanidins, and isoflavones (36). Apart from their antioxidative properties, the potential mechanism of action of flavonoids is their interaction with neuronal signaling cascades such as PI3K/Akt, protein kinase C, and MAPK which leads to decreased apoptosis and enhanced neuronal survival (37). Additionally, flavonoids induce angiogenesis and neurogenesis and act directly to counteract neurotoxic and proinflammatory agents (38). Moreover, flavonoids exert antioxidant effects by scavenging free radicals and reactive oxygen species (ROS) (39, 40). As oxidative stress is thought to be the leading cause of dopaminergic neuronal loss in the substantia nigra, neuroprotective molecules that ameliorate oxidative stress and excitotoxicity are of primary importance (41-43).
Camelia sinesis (Green tea) is a shrub native to Asia. Green tea is widely consumed as a beverage in Japan, China, and other Asian nations. Tea flavonoids exert antioxidant, anti-inflammatory and neuroregenerative effects (44, 45). Epicatechin-3-gallate (EGCG) is the tea’s most abundant polyphenol which offers antioxidant and neurogenerative effects (46-48). Epidemiological studies showing the alleviation of PD risk in tea drinking populations and its low prevalence in Chinese populations provides additional support for its neuroprotective effects in PD (49-51). In vivo studies in MPTP-induced parkinsonian mice have shown that green tea extract can attenuate DA depletion and dopaminergic neuronal survival in the substantia nigra region of the brain (51). The catechol-like structure of EGCG exerts an inhibitory effect on DA uptake by blocking uptake of the neurotoxin MPP+ (1-methyl-4-phenylpyridinium) and protecting dopaminergic neurons against MPP+ injury (52). Moreover, EGCG regulates extracellular signaling kinases (ERK1/2 and mitogen activated protein kinases), the major impediment to neuronal damage and oxidative stress (53). Additionally, the metal chelating ability of green tea flavonoids can attenuate iron dyshomeostasis observed in PD (54). Supplementation with green tea extract also mitigates NF-kB immunoreactivity and oxidative stress induced in SHSY-5Y cells (55).
Withania somnifera (Ws) is an Indian Ayurvedic traditional medicinal herb grown in India, Africa, and the Mediterranean region. The root extract is rich in steroidal lactones including withanone, withaferin, withanolides, withasomidienone, and withanolide (56). These compounds have been reported to inhibit metastasis and quinone reductase activity, .and preferentially affect the cholinergic signal transduction cascade of the cortical and basal forebrain, and thus may be beneficial for the treatment of PD. One study recently showed that withanolides are potent suppressors of NF-κB activation mediated by a number of inflammatory agents, and that this suppression occurs through inhibition of IκBα kinase (IKK) complex consisting of IKK-α, IKK-β, IKK-γ (also called NEMO), IKK-associated protein 1, FIP-3 (type 2 adenovirus E3-14.7-kDa interacting protein), heat shock protein 90, and glutamic acid, leucine, lysine, and serine–abundant protein. Experimental studies have also shown that MPTP-administered parkinsonian mice showed improvement in neuronal survival and locomotion after administration of Withania somnifera (Ws) root extract. Importantly, there was a significant increase in catacholamines such as DA, glutathione (GSH), and glutathione (GSH) peroxidase enzyme in Ws ingested PD mice compared to control. Also, there was a remarkable increase in the level of DA metabolites, 3,4- dihydroxy-phenylacetic acid (DOPAC) and homovanillic acid (HVA) (57). Further evidence supporting the antioxidative effect of Ws root extract against PD mice is notable. Treatment with Ws root extract (100mg/kg) for a period of 7 days correlated with a significant reduction in aggravated levels of malondialdehyde, superoxide, and catalase in the brains of PD mice (58). The ability of Ws to alleviate gait disorders, inflammation, and brain aging and its potential to upregulate p13 kinase and enhance neuronal growth has also been numerously reported (59, 60).
Ginseng is an adaptogen that resists adverse effects of harmful substances, restoring homeostasis and acting as a psychic energizer (61, 62). Scientific evidence has substantiated the neuroprotective potential of ginseng extract G115 against dopaminergic neuronal loss and gait disturbance in PD mice models (63). Ginsenosides are triterpinoid saponins unique to Panax ginseng species distributed throughout China, North and South Korea, and Russia. These compounds elicit a pleotrophic mechanism of action. The DA uptake inhibiting activity of that ginsenosides have the potential to act as NMDA antagonists and protect neurons from mitochondrial dysfunction and glutamate elevation and excitotoxicity caused by MPTP (64, 65). The potential of ginsenosides to reduce calcium influx and free radical generation and oxidative stress may also play a role in ginseng’s disease modifying effects (66, 67).
Caffeine is an Adenosine 2A receptor antagonist present in coffee beans from the Coffea arabica and Coffea canephora plants, which are widely distributed in Asia and Africa. Caffeine exerts neuroprotective effects against dopaminergic neuronal loss in MPTP-induced PD mice (68-70). Furthermore, caffeine causes reversal of motor deficit in PD mice models (71). Caffeine’s behavioral and neurobiochemical effects have been shown to cause a reduction in apomorphine-induce rotation and enhanced motor function. In experimentally depletion of dopaminergic neurotransmssion using neurotoxic, 6-hydroxydopamine (6-OHDA), the level of DA and its metabolites have also been shown to recover following caffeine administration (72).
Ginkgolides and bilobalides are unique phytochemicals present only in the Ginkgo biloba tree (73). EGb761, a well-defined mixture of active compounds extracted from its leaves, exerts a protective effect against oxidative stress induced by MPTP in C57BL/6J mice. Mice receiving EGb761 recovered striatal DA levels and tyrosine hydroxylase in the striatum and substantia nigra pars compacta. The neuroprotective effect of EGb761 against MPTP neurotoxicity is associated with its free radical scavenging activity, blockade of lipid peroxidation, and reduction of superoxide radical production (74). Furthermore, G. biloba extract exhibits inhibitory effects on MAO activity on rat mitochondria (75), suggesting that its neuroprotective effects on dopaminergic neurons may be due to its inhibitory action on MAO.
Polygala root extract (PRE) consists of xanthones, saponins, and oligosaccharide esters (76) and is reported to have as neuroprotective effect on dopaminergic neuron in 6-OHDA induced neurotoxicity in both in vitro and in vivo PD models. The possible mechanism of action is due to reduced ROS and nitric oxide (NO) production and altered caspase-3 activity (77). Moreover, oligosaccharide derivatives of PRE act against clinical depression by binding to norepinephrine transporter proteins (78). Furthermore, the 3,4,5-trimethoxycinnamic acid (TMCA) present in PRE exerts anti-stress effects through suppression of norepinephrine (79).
Baicalein and baicalin are flavonoids found in large concentrations in the Scutellaria baicalensis and Bupleurum scorzonerifolfium (S/B) herbs (80). These compounds have been shown to attenuate iron-induced lipid peroxidation and DA depletion in the substantia nigra. They can also augment GSH levels, hinder α- synuclein aggregation, and reduce iron-induced mitochondrial stress and apoptosis. The 3-OH group attached to the benzene ring of the molecular target baicalein is responsible for α-synuclein disaggregation. A significant reduction in nitric oxide synthase and inflammatory markers was also observed in the substantia nigra of neurotoxic rat brain treated with S/B remedies. Furthermore, baicalein ameliorated 6-OHDA induced toxicity by decreasing phosphor-JNK and caspase activity in SHSY-5Y cells (81). Baicalein pretreatment improved behavior in MPTP induced PD mice. The potential mechanism of action may rely on increased DA and melatonin (5-HT) in the striatum (82).
Pueraria thomsonii Benth is a herbal medicine enriched with isoflavonoids such as puerarin, daidzin, daidzein, and genistein (83). The neuroprotective effect of genistein in dopaminergic neurons has been reported, where in 6- OHDA induced nerve growth factor (NF) differentiated pc12 cells, genistein and daidzin showed an inhibitory effect on caspase 3 and caspase 8 activation, thereby preventing apoptosis (84).
Uncaria rhynchophylla is used as a traditional medicine to treat convulsive disorders, tremors, and hypertension (85). The major alkaloids are rhynchophylline, corynoxeine, corynantheine, and hirsutine and the major flavonoids are catechin and epicatechin. All have been shown to have a cytoprotective effect (86). In animal with depleted DA activity using 6- OHDA Uncaria rhynchophylla extract (URE) ameliorated dopaminergic neuronal loss and apomorphine induced rotation. Meanwhile, a significant reduction was observed in ROS generation and caspase 3 activity and a remarkable maintenance of cell viability and GSH levels was observed in neurotoxic PC12 cells.
Polygonum cuspidatum is a perennial herb used primarily in Traditional Chinese Medicine and other Asian cultures. Recent studies have shown the neuroprotective potential of P. cuspidatum-derived resveratrol (RES) in 6-OHDA induced mice. The protective effect is exerted by its antioxidant reducing and antiapoptotic abilities (87). In another study, male Wistar rats pretreated with RES escaped dopaminergic neuronal loss and neurobehavioral defects following 6- OHD injection. This affect is likely due to upregulation of antioxidant enzyme status and mitigation of DA deprivation (88).
Bacopa monniera is a traditional medicinal plant used extensively to treat anxiety, epilepsy, and memory deficits (89). It has been substantiated that Bacopa moniera extract (BME) exerts a dose dependent protective effect in 6-OHDA-lesioned PD rat models as determined by significant improvements behavioral activity and restoration of GSH, SOD, and catalase activity levels and reduced lipid peroxidation (90). The potential mechanism of action of BME is attributed to its antioxidant, free radical scavenging properties, and DA-enhancing effects (91).
Cassia obtusifolia L. is an annual plant commonly consumed as roasted tea and is widely distributed in Korea and China. Cassiae semen (sicklepod) seed extract (CSE) has been shown to protect against dopaminergic neuronal degeneration in the substantia nigra and striatum of MPTP-induced PD mice models and dopaminergic neurons in vitro. In 6-OHDA induced pc12 cells, CSE supplementation has been demonstrated to mitigate cell damage and attenuate ROS generation and mitochondrial membrane depolarization. MPP+, the neurotoxic metabolite of MPTP, induces dopaminergic neuronal loss by inhibiting the activity of respiratory complex 1 in the mitochondria of dopaminergic neurons (92).
Fruit, Vegetable and Spice derived Nutraceuticals for PD
Levodopa (L-DOPA) was first isolated from the seeds of the leguminous plant Mucuna pruriens, which is native to India and other parts of the tropics including Central and South America (93, 94) and Mucuna spp are considered to be richest natural sources of L-DOPA hotels of the world bay of many coves resort, luxury logdes of new zealand . (95). The administration of 30 grams of Mucuna seed powder has been reported to have antiparkinsonian effects in PD patients, with a more rapid onset of action, shorter latency, and enhanced improvement compared to the standard combination of levodopa (200 mg) and carbidopa (50mg) treatments (96). Vicia faba (broad bean) is an edible bean found in the Mediterranean region and is rich in L-DOPA (97). The ingestion of broad beans in PD patients has been shown to elevate L-DOPA plasma levels which have correlated with cognitive improvement in the same magnitude as levodopa/carbidopa administration (98, 99). Another study suggested that 2,4- epibrassinolide (24-Epi), a natural Brassinosteroid found in V. faba, offers antioxidative and antiapoptotic effect on MPTP induced PC12 cells. The mechanism underpinning the effect was considered to be due to modulation of antioxidant enzymes, Bax/Bcl-2 protein ratio, and cleaved caspase-3.
Chaenomeles speciosa is an East Asian native shrub that is known for its nutritionally edible fruit; the common flowering quince (FQ). FQ is used traditionally to treat neuralgia, migraine, depression, tremors, and dyskinesia (100, 101). Scientific evidence has proven FQ to be a potent DA transport (DAT) inhibitor that can be used as a therapeutic target for PD. In vitro studies have shown that FQ administration attenuates DA uptake by DATs in Chinese hamster ovary (CHO) cells expressing DAT (D8 cells). Additionally, FQ maintains cell viability, tyrosine hydroxylase (TH) activity, and behavioral performance in MPTP induced neurotoxic models of PD.
The rhizome of the perennial Cyperus rotundus is used as a functional food and medicine in Korea. Reports have suggested that cyperi rhizome extract (CRE) exerts protective effect on 6-OHDA induced PD models. CRE mitigates ROS and NO production and intensifies cell viability and caspase 3 activity. It also modulates mitochondrial membrane potential and dopaminergic neuronal loss in neurotoxic cell cultures (102).
Extracts of the edible fruit of the deciduous Morus alba L. (mulberry) have been demonstrated to have neuroprotective efficacy in in vitro and in vivo PD models, with the efficacy attributed to its content of polyphenols, anthocyanin, rutin, quercetin and α and γ tocopherol (103). In 6-OHDA mediated neurotoxic cell lines, the mulberry extract (ME) elicits antioxidant and antiapoptotic effects. Biochemical assays have shown that ME can stabilize the mitochondrial membrane, and regulate the expression of Bcl-2, Bax, and caspase 3 proteins involved in apoptosisAdministration of ME can also alleviate bradykinesia and dopaminergic neuronal damage in the substantia nigra in vivo (104, 105). Citrus is a family of flowering plants ranging from herbs, shrubs, and trees, including Citrus sinensis (orange), C. lemon (lemon), and C. paradisi (grape fruit). Citrus flavonoids are composed of flavonones, flavone glycosides and polymethoxyflavones (106). It has been reported that polymethoxyflavones present in orange and lemon peel can mitigate the loss of dopaminergic neurons and tyrosine hydroxylase in the substantia nigra of the 6- OHD rat model (107). Similar effects exerted by the flavonoid, naringenin, which is highly present in citrus, in PD models have also been noted (108). Naringenin and another citrus flavonoid, hesperidin attenuates LPS/IFNγ-induced TNF-α production in glial cells and naringenin also represses LPS/IFN-γ induced iNOS expression, p38 mitogen-activated protein kinase (MAPK) phosphorylation, and transcription-1 (STAT-1) which are associated with microglial and astrocyte-mediated inflammatory response (109).
Vitis vinifera (grape) is one of the world’s largest fruit crops and is enriched with catechins, epicatechins, anthocyanins, and resveratrol. Grape juice (GJ) administration has been correlated with a decrease in behavioral deficits in 6-OHDA induced mice (110). Resveratrol has been demonstrated to protect against MPTP induced apoptosis in neuronal cells, with the underpinning mechanism considered to involve the modulation of the expression of pro-apoptotic Bcl-2 gene and antiapoptotic Bax gene. Resveratrol administration has been shown to attenuate MPTP induced mitochondrial release of cytochrome c and caspase 3 activation, and facilitate membrane stabilization and neuronal survival (111). Interestingly, oxyresveratrol, a stilbinoide, contains one extra OH group and is more effective than resveratrol against 6-OHDA toxicity in SHSY-5Y cells. This is probably due to a greater antioxidant potential, more potent inhibition of the JNK pathway and an increase in activity of the nuclear sirtuin enzyme (SIRT1) which is associated with longevity (112).
Allium sativum (garlic), the edible bulb of the Liliacea family, possesses innumerable health benefits. S- allylcysteine (SAC), its most abundant organic sulfur containing compound, possesses radical scavenging activity (113) which when administered to MPTP induced neurotoxic mice improved locomotor function which correlated with increased dopamine production, alleviated lipid peroxidation and superoxide production, and enhanced superoxide dismutase (SOD) activity (114).
Morin (3,5,7,20,40-pentahydroxyflavone) is a bioflavonoid found in Prunus dulcis (almond), Maclura pomifera (osage orange), Maclura tinctoria (old fustic), and in leaves of Psidium guajava (guava) (115). The health benefits of morin have been investigated in in vitro and in vivo PD models. In PC12 cells, morin supplementation at 5-50μmol/L significantly viability losses, apoptosis, and ROS generation following MPTP treatment. In MPTP-induced PD mice models morin administration attenuated behavioral deficits and DA deprivation (116).
Lycopersicon esculentum L. (tomato) is rich in lycopene and well-known for its edible fruit. Pretreatment with a diet containing lyophilized tomato powder correlated with a decreased DA loss in a MPTP- induced PD model (117).
Black cumin (Nigella sativa)seeds are commonly used in culinary preparations. Thymoquinone, its main bioactive component, has been shown to protect against dopaminergic neuronal deprivation in MPP+ and rotenone induced neurotoxic PD models. In primary dopaminergic neuronal cell lines, long term and short term toxicity were reversed by thymoquinone treatment (118).
Sesame indicum is a flowering plant native to Africa, with high production in India and China. Sesame seed and its oil contain the bioactive molecules sesamin, sesamol, and sesaminol. Sesamin is a lipophilic lignin offering moderate antioxidant and immunomodulatory effects (119, 120). Sesamin has been demonstrated to have a DA enhancing effect in rotenone-induced loss of dopaminergic neurons in mice. Interestingly, MPP+ treated neuronal PC12 cells exhibit a significant decrease in ROS generation and oxidative stress following sesamin treatment. Sesamin modulates the expression of tyrosine hydroxylase (TH), SOD, and catalase, impedes inducible NO synthase (iNOS) protein expression in neuronal cells, and lowers mRNA levels of the potent pro-inflammatory cytokine interleukin-6 (IL-6) in microglial cells (121).
Curcuma longa is a rhizome grown throughout India and is extensively used in food additives and medicines (122). Its diverse cytoprotective action is offered by polyphenolic curcuminoids, consisting of curcumin, demethoxy curcumin (DMC), and bis-demethoxy curcumin (BDMC). In vitro and in vivo models of PD have shown that curcumin demonstrates a disease modifying effect by protecting dopaminergic neurons against LPS and α-synuclein induced neurotoxicity, mitigating DA loss, which allieviates oxidative stress and limits mitochondrial dysfunction (123, 124). The MPTP- mediated depletion of DA and TH immunoreactivity in dopaminergic neurons was reverted following the addition of curcuminoids. The administration of curcuminoids suppressed over-expression of iNOS, reduced pro-inflammatory cytokines, and total nitrite generation in the striatum of MPTP-intoxicated mice (125, 126). Another study has shown that curcumin protects against dopaminergic neurotoxicity induced by MPTP or MPP+ in C57BL/6 N mice and SHSY-5Y cells by inhibiting the JNK pathway (127).
Zingiber officinale (ginger) is a major spice that exhibits neuroprotective effects. Zingerone and 6 shogaol compounds isolated from ginger, impede 6-OHD induced DA loss in mouse striatum (128) and prevent apoptotic neural cell death (129). The DA replacement effect is possibly through up-regulation of the superoxide scavenging activity (SOSA) of SOD. The lipid peroxidation lowering effect of zingerone is also considered to contribute to its cytoprotective effect (130).
Neuroprotective Effects of Ornamental Plants
Paeoniae lactiflora is an ornamental flowering plant known for its dried root. Paeoniflorin (PF), a monoterpene glucoside, is abioactive molecule produced by Paeoniae alba Radix (131) and is reported to have a neuroprotective effect on dopaminergic neurons in an MPTP mouse model for PD. Dose dependent subcutaneous administration of PF restored TH positive cells, dopaminergic neuronal paucity, and ameliorated bradykinesia. The mechanism of action is due to PF acting as an agonist for the adenosine A1 receptor, which down regulates microglial and astrocytic activation and neuroinflammation (132).
Dendrobium nobile Lindl. is an ornamental medicinal plant in the Orchidacea family, which is rich in the bioactive benzyl compound chrysotoxine (133).
Chrysotoxine is reported to protect SHSY-5Y cells against 6-OHDA toxicity through mitochondrial protection and NF-κB modulation. Chrysotoxine pretreatment exerts beneficial effects including attenuation of 6-OHDA- induced intracellular generation of ROS, and activation of p38 MAPK and ERK1/2. Mitochondrial dysfunction is reverted through multiple mechanisms including the decrease of membrane potential, increase of intracellular free Ca2+, release of cytochrome c, imbalance of Bax/Bcl- 2 ratio and decrease in activation of caspase-3. An anti- inflammatory for response is exerted by suppression of NF- κB activation by blocking its translocation to the nucleus, thereby preventing up-regulation of iNOS and intracellular NO release (134).
Rosmarinus officinalis (rosemary) is an aromatic ornamental plant rich in cytoprotective biomolecules. R. officinalis extracts have been shown to offer neuroprotective potential in a model for H2O2-induced apoptosis of human dopaminergic cells correlating with suppression of of the expression of Bax, caspase-3 and -9 and Bcl-2 genes (135). Rosmaric acid (RA) has been demonstrated to have traiter l’anxiete, pour la sedation avant et apres l’anesthesie effect in a 6- OHDA-lesioned rat model of PD. RA exerts its effect by decreasing iron levels in the substantia nigra and regulating the ratio of Bcl-2/Bax gene expression (136). Carnosol, another major rosemary component has been shown to offer protection against rotenone-induced neurotoxicity by promoting cell viability and down regulating caspase 3 action, withincreased TH, Nurr1, and extracellular signal-regulated kinase 1/2 also likely to be involved (137).
Bioactive molecules Derived from Parasitic Plants and Fungi
Cistanche salsa is a parasitic plant used as a Chinese traditional medicine containing acteoside, echinacoside, and tubuloside as its major phenylethanoid glycosides (PHGs). C. salsa extracts containing these bioactive molecules have been shown to restore behavioral deficit and dopamine depletion in strata of MPTP induced C57 mice (138). They has also been demonstrated to protect dopaminergic neurons in the substantia nigra of PD model mice, which correlated with neurobehavior improvements (climbing test) and TH positive neuronal levels (139).
Gastrodia elata (GE) blume is a saprophytic perennial herb used traditionally as a medicine due to its wide range of therapeutic benefits. Protective effects of GE extract against MPP+-induced cytotoxicity in human dopaminergic SHSY-5Y cells demonstrated that GE extract dose dependently improved cell viability maintenance, attenuated oxidative damage, modulated expression of Bcl-2 and Bax, caspase-3, and limited poly(ADP-ribose) polymerase proteolysis (140). Recently, MPP+ induced MN9D dopaminergic cells were shown to exhibit antiapoptotic effects following GE extract treatment. The v anillyl alcohol present in GE was considered to act by attenuating the MPP+ induced elevation of ROS levels and decreasing the Bax/Bcl-2 ratio and poly (ADP-ribose) polymerase (141).
Ganoderma lucidum (GI) is a woody mushroom widely used as an alternative medicine. One study has identified that the neuromodulatory potential of GL on dopaminergic neurons to protect against inflammatory damage induced by microglial activation following exposure to MPP+ and LPS. The mechanism of action is purportedly the ability of GL to protect against the production of the microglia-derived toxic factors NO, TNF-α, IL-1ß, and superoxide (142).
Current and Future Developments
Current pharmacotherapies for PD do not provide the much desired permanent curative benefits to patients. Scientists in the field of nutrition have identified natural products as potential adjuvant treatments to conventional drug therapy to attenuate the PD symptoms and reduce the dose of anti-Parkinson drugs and the incidence of associated adverse events. There is a high rate of dyskinesia and relapse of Parkinsonian symptoms following long-term treatment with levodopa (143). Combination therapy with natural herbal products has demonstrated substantial benefits in lowering levodopa- related complications (144). However, the long term effect of combination therapy has not been investigated, and potential interactions with drugs that are currently used in the PD treatment remain unclear. The dosage regimen for anti-parkinson’s drug is of major clinical importance of anti-parkinson’s drug due to its lifelong requirement. Adjunct therapy with natural products may ultimately prove useful for reducing the dose of levodopa for managing PD symptoms.
While many natural products appear promising for the treatment of PD and management of dyskinesia, a global initiative to standardize compositions for pharmacological applications is essential to ensure good quality and efficacy. The efficacy of natural formulations are limited due to several problems related to the original species of individual herbs, chemical compositions and indexes, preparations and indications for herbal products (145). Chinese formulas currently available on the market are composed of a variety of natural products and there exists wide heterogeneity between the natural products described in this review. Standardization of single and combinations of natural products in human clinical trials will be a major factor for translating preclinical data to the clinical setting.
Future clinical trials using natural products for the treatment of PD need to be improved methodologically to ensure good reproducibility, to improve clinical benefit, and to reduce the potential to cause harm. Double blinded studies and the inclusion of placebos should be employed in the study design. Protocols need to be defined prior to commencement of clinical trials to guarantee the transparency of the study finding(s) and prevent publication bias (146).
Prior to the onset of modern medicine, societies all over the world have employed various natural products as ‘food for medicine’. With hegemony of biomedical sciences with its reductionism approach, the usage of natural products for the cause of prevention and amelioration of illness was deemed a relic of the past. The idea encapsulating ‘food for medicine’ was often portrayed as Grandmother’s medicine. However, despite decades of the reductionism approach in health and medicine, some of the emerging prevalent conditions such as PD appear to be inaccessible to modern medical interventions. In the age of paradigm shift, interests towards natural product have been rekindled. Rather than being limited to grandmother’s empiricism, development of science has rendered the possibility of scrutinizing mechanisms of natural project with lances of modern scientific approach. The evidence suggesting the heuristic value of some of the natural products is abundant. This paper has specifically explored the role of herbs, fruit, vegetable and spice, ornamental plants and parasitic plants and fungi in the trajectory of PD. The evidence appears to be tenderized with notion that the wisdom of Grandmother’s medicine is not a relic of the past; the natural product is once again live and kicking. If science would progress on such a quest, natural products may address the underlying pathological processes associated with the development and progression of neurodegenerative diseases, and PD in particular.
Acknowledgements: The project was supported by Sultan Qaboos University; Oman in the form of internal grant is gratefully acknowledged (IG/AGR/FOOD/14/01) and also supported by the Research Council; Oman (Grant # RC/AGR/FOOD/11/01). Nady Braidy is the recipient of an Alzheimer’s Australia Viertel Foundation and National Health and Medical Research Council Early Career Postdoctoral Research Fellow at the University of New South Wales.
Conflict of interest: Author has no conflict of interest with this paper.
1. de Lau LM, Breteler MM. Epidemiology of Parkinson’s disease. Lancet Neurol 2006;5:525–35.
2. Tanner CM, Goldman SM. Epidemiology of Parkinson’s disease. Neurol Clin 1996;14:317-335.
3. Hoehn MM, Yahr MD. Parkinsonism: onset, progression and mortality. Neurology 1967;17:427-42.
4. Shulman LM, Taback RL, Bean J, Weiner WJ. Comorbity of the nonmotor symptoms of Parkinson’s disease. Movement Disorders 2001;16:507–510.
5. Al-Adawi S, Dawe GS, Al-Hussaini A. Aboulia: Neurobehavioral dysfunction of dopaminergic system? Medical Hypotheses 2000;54:523-530.
6. Pollanen MS, Dickson DW, Bergeron C. Pathology and biology of the Lewy body. J Neuropathol Exp Neurol 1993;52:183-191.
7. Kuzuhara S, Mori H, Izumiyama N, Yoshimura M, Ihara Y. Lewy bodies are ubiquitinated: a light and electron microscopic immunocytochemical study. Acta Neuropathol (Berl) 1988;75:345-353.
8. Forno LS. Neuropathology of Parkinson’s disease. J. Neuropathol. Exp. Neurol.1996;55:259–72.
9. Kruger R, Kuhn W, Müller T, Woitalla D, Graeber M, Kösel S, Przuntek H, Epplen JT, Schöls L, Riess O. Ala30Pro mutation in the gene encoding alpha- synuclein in Parkinson’s disease. Nat Genet 1998;18:106-08.
10. Kitada T, Asakawa S.Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism. Nature 1998;392:605- 608.
11. Menegon A, Board PG. Blackburn AC, Mellick GD, Le Couteur DG. Parkinson’s disease, pesticides, and glutathione transferase polymorphisms. Lancet 1998;352:1344 –46.
12. Perry G, Cash AD, Smith MA. Alzheimer disease and oxidative stress. J Biomed Biotechnol 2002; 2:120 –3.
13. Perry G, Taddeo MA, Nunomura A, Zhu X, Zenteno-Savin T, Drew KL, Shimohama S, Avila J, Castellani RJ, Smith MA. Comparative biology and pathology of oxidative stress in Alzheimer and other neurodegenerative diseases: beyond damage and response. Comp Biochem Physiol C Toxicol Pharmacol 2002; 133:507–13.
14. Al-Maskari MY, Hanif MA, Al-Maskri AY, Al-Adawi S. Basil: A Natural Source of Antioxidants and Neutraceuticals (Editors): M. Mohamed Essa, A. Manickavasagan, In: Natural Products and their Active Compounds. Nova Science Publishers, Inc, 2011.
15. Essa MM, Vijayan RK, Castellano-Gonzalez G, Memon MA, Braidy N, Guillemin GJ. Neuroprotective effect of natural products against Alzheimer’s disease. Neurochem Res 2012;37:1829-42.
16. Fulton B, Benfield P. Galantamine. Drugs Aging 1996;1:60–65.
17. Peter J. Houghton PJ, Howes MJ. Natural Products and Derivatives Affecting Neurotransmission Relevant to Alzheimer’s and Parkinson’s Disease. Review Neurosignals 2005;14:6–22.
18. Carlsson A. “A paradigm shift in brain research,” Science 2001;294:1021-24.
19. Daubner SC, Le T, Wang S. Tyrsoine hydroxylase and regulation of dopamine synthesis. Arch Biochem Biophys 2011; 508:1-12.
20. Moskaug J, Carlsen H, Myhrstad MCW, Blonhoff R. Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 2005;81:2775-2835
21. Bergmark J, Carlsson A, Granerus AK, Rudolf Jagenburg R, Magnusson T, Svanborg A. et al. Decarboxylation of orally administered L-dopa in the human digestive tract. Naunyn-Schmiedebergs Arch Pharmacol 1972;272:437.
22. Manyam BV, Dhanasekaran M, Hare TA. Effect of antiparkinson drug HP-200 (Mucuna pruriens) on the central monoaminergic neurotransmitters. Phytother Res 2004;18:97–101.
23. Ogawa N, Tanaka KI, Asanuma M, Kawai M, Masumizu T, Kohno M, Mori A. Bromocriptine protects mice against 6-hydroxydopamine and scavenges hydroxyl free radicals in vitro. Brain Research 1994;657:207–13.
24. Schapira AHV. Neuroprotection in PD—a role for dopamine agonists?. Neurology. 2003:61:S34–S42.
25. Kitamura Y, Taniguchi T, Shimohama S, Akaike A, Nomura Y. Neuroprotective mechanisms of antiparkinsonian dopamine D-receptor subfamily agonists. Neurochemical Research 2003;28:1035–40.
26. Kebabian JW, Calne DB. Multiple receptors for dopamine.Nature 1979;277:93–96.
27. Missale C, Nash SR, Robinson SW, Jaber M, Caron MG. Dopamine receptors: from structure to function. Physiol Rev 1998;78:189–225.
28. Sokoloff P, Giros B, Martres MP, Bouthenet ML, Schwartz JC. Molecular cloning and characterization of a novel dopamine receptor (D3) as a target for neuroleptics. Nature 1990;347:146–151.
29. Melzig MF, Putscher I, Henklein P, Haber H. In vitro pharmacological activity of the tetrahydroisoquinoline salsinol present in products from Theobroma cacao L. like cocoa and chocolate. J Ethnopharmacol 2000;73:153–159.
30. Waldmeier PC. Amine oxidases and their endogenous substrates (with special reference to monoamine oxidase and the brain). Journal of Neural Transplantation Supplement 1987;23:55-72.
31. O’Brien EM, Kiely KA, Tipton KF. A discontinuous luminometric assay for monoamine oxidase. Biochemical Pharmacology1993;46:1301-1306.
32. Schwarz MJ, Houghton PJ, Rose S, Jenner P, Lees AD. Activities of extract and constituents of Banisteriopsis caapi relevant to parkinsonism. Pharmacol Biochem Behav 2003;75:627–33.
33. Van Acker SA, van den Berg DJ, Tromp MN, Griffioen DH, Van Bennekom Wp, Van Der Vijgh WJ, Bast A. Structural aspects of antioxidant activity of flavonoids. Free Radic Biol Med 1996;20:331–42.
34. Schroeter H, Spencer JP, Rice-Evans C, Williams RJ. Flavonoids protect neurons from oxidized low-density-lipoprotein-induced apoptosis involving c-Jun N- terminal kinase (JNK), c-Jun and caspase-3. Biochem J 2001;15:358(Pt 3):547-57.
35. Smith JV, Burdick AJ, Golik P, Khan I, Wallace D, Luo Y.Anti-apoptotic properties of Ginkgo biloba extract EGb 761 in differentiated PC12 cells. Cell Mol Biol (Noisy-le-grand) 2002;48:699-707.
36. Manach C, Scalbert A, Morand C, Rémésy C, Jiménez L. Polyphenols: foodsources and bioavailability. Am J Clin Nutr 2004;79:727–47.
37. Spencer JPE. The impact of flavonoids on memory: physiological and molecular considerations. Chem Soc Rev 2009;38:1152–61.
38. Vafeiadou K, Vauzour D, Spencer JP. Neuroinflammation and its modulation by flavonoids. Endocr Metab Immune Disord Drug Targets 2007;7:211–24.
39. Rice-Evans CA, Miller NJ, Paganga G. Structure antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996;20:933–56.
40. Pollard SE, Kuhnle GG, Vauzour D, Vafeiadou K, Tzounis X, Whiteman M, Rice- Evans C, Spencer JP. The reaction of flavonoid metabolites with peroxynitrite. Biochem Biophys Res Commun 2006;350:960–68.
41. Schapira AH, Olanow CW. Neuroprotection in Parkinson disease: mysteries, myths, and misconceptions. JAMA 2004;291:358–64.
42. Kedar NP. Can we prevent Parkinson’s and Alzheimer’s disease? J Postgrad Med 2003;49:236–45.
43. Dawson TM, Dawson VL. Molecular pathways of neurodegeneration in Parkinson’s disease. Science 2003;302:819–22.
44. Anandhan A, Tamilselvam K, Radhiga T, Rao S, Essa MM, Manivasagam T. Theaflavin, a black tea polyphenol, protects nigral dopaminergic neurons against chronic MPTP/probenecid induced Parkinson’s disease. Brain Res 2012;1433:104- 13.
45. Anandhan A, Essa MM, Manivasagam T. Therapeutic Attenuation of Neuroinflammation and Apoptosis by Black Tea Theaflavin in Chronic MPTP/Probenecid Model of Parkinson’s Disease. Neurotox Res 2013;23:166-73.
46. Slikker W, Youdim MB, Palmer GC, Hall E, Williams C, Trembly B. The future of neuroprotection. Ann NY Acad Sci 1999;890:529-33.
47. Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice-Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys 1995;322:339 –46.
48. Nanjo F, Goto K, Seto R, Suzuki M, Sakai M, Hara Y. Scavenging effects of tea catechins and their derivatives on 1,1-diphenyl-2picrylhydrazyl radical. Free Radic Biol Med 1996;21:895–902.
49. Checkoway H, Powers K, Smith-Weller T, Franklin GM, Longstreth WT Jr, Swanson PD. Parkinson’s disease risks associated with cigarette smoking, alcohol consumption, and caffeine intake. Am J Epidemiol. 2002;155:732–8.
50. Tan EK, Tan C, Fook-Chong SM, Lum SY, Chai A, Chung H, Shen H, Zhao Y, Teoh ML, Yih Y, Pavanni R, Chandran VR, Wong MC. Dose-dependent protective effect of coffee, tea, and smoking in Parkinson’s disease: a study in ethnic Chinese. J Neurol Sci 2003;216:63–7.
51. Levites Y, Weinreb O, Maor G, Youdim MBH, Mandel S. Green tea polyphenol (_)-epigallocatechin-3-gallate prevents N-methyl-4phenyl-1,2,3,6- tetrahydropyridine-induced dopaminergic neurodegeneration. J Neurochem 2001; 78:1073–82.
52. Deleu D, Northway MG, Hanssens Y. Clinical pharmacokinetic and pharmacodynamic properties of drugs used in the treatment of Parkinson’s disease. Clin Pharmacokinet 2002;41:261–309.
53. Satoh T, Nakatsuka D, Watanabe Y, NagataI, Kikuchi H, Namura S. Neuroprotection by MAPK/ERK kinase inhibition with U0126 against oxidative stress in a mouse neuronal cell line and rat primary cultured cortical neurons. Neurosci Lett 2000;288:163–6.
54. Guo Q, Zhao B, Li M, Shen S, Xin W. Studies on protective mechanisms of four components of green tea polyphenols against lipid peroxidation in synaptosomes. Biochim Biophys Acta 1996;1304:210 –22.
55. Levites Y, Youdim MBH, Maor G, Mandel S. Attenuation of 6-hydroxydopamine (6-OHDA)-induced nuclear factor-kappaB (NFkappaB) activation and cell death by tea extracts in neuronal cultures. Biochem Pharmacol 2002;63:21–9.
56. Mishra LC, Singh BB, Dagenais S. Scientific basis for the therapeutic use of Withania somnifera (ashwagandha): a review. Alternative Medicine Review 2000;5:334–46.
57. RajaSankar S, Manivasagam T, Sankar V, Prakash S, Muthusamy R, Krishnamurti A, Surendran S. Withania somnifera root extract improves catecholamines and physiological abnormalities seen in a Parkinson’s disease model mouse. Journal of Ethnopharmacology 2009;125:369–73.
58. RajaSankar S, Manivasagam T, Surendran S. Ashwagandha leaf extract: A potential agent in treating oxidative damage and physiological abnormalities seen in a mouse model of Parkinson’s disease. Neuroscience Letters 2009;454:11–15.
59. Singh RH, Narsimhamurthy K, Singh G. Neuronutrient impact of Ayurvedic Rasayana therapy in brain aging. Biogerontology 2008;9:369–374.
60. Surendran S. Rotavirus infection: molecular changes and pathophysiology. Experimental and Clinical Sciences International Online Journal for Advances in Science 2008;7:154–162.
61. Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical Biology and Medicine. 1999;27:612–16.
62. Lian XY, Zhang Z, Stringer JL. Protective effects of ginseng components in a rodent model of neurodegeneration. Annals of Neurology 2005;57:642–648.
63. Kampen JV, Robertson H, Hagg T. Neuroprotective actions of the ginseng extract G115 in two rodent models of Parkinson’s disease. Experimental Neurology. 2003;184:521–29.
64. Beal MF, Brouillet E, Jenkins B, Henshaw R, Rosen B, Hyman BT. Age-dependent striatal excitoxic lesions produced by the endogenous mitochondrial inhibitor malonate. J Neurochem 1993;61:1147–50.
65. Brouillet E, Beal MF. NMDA antagonists partially protect against MPTP induced neurotoxicity in mice. Neuroreport 1993;4:387–390.
66. Kitts DD, Wijewickreme AN, Hu C. Antioxidant properties of a North American ginseng extract. Mol Cell Biochem 2000;203:1–10.
67. Wang CN, Shiao YJ, Kuo YH, Chen CC, Lin YL. Inducible nitric oxide synthase inhibitors from Saposhnikovia divaricata and Panax quinquefolium. Planta Med 2000;66:644–47.
68. Fisone G, Borgkvist A, Usiello A. Caffeine as a psychomotor stimulant: mechanism of action. Cell Mol Life Sci. 2004;61:857–72.
69. Schwarzschild MA, Chen JF, Ascherio A. Caffeinated clues and the promise of adenosine A(2A) antagonists in PD. Neurology 2002;58:1154–60.
70. Chen JF, Xu K, Petzer JP, Staal R, Xu YH, Beilstein M, Sonsalla PK, Castagnoli K, Castagnoli N Jr, Schwarzschild MA. Neuroprotection by caffeine and A(2A) adenosine receptor inactivation in a model of Parkinson’s disease. J Neurosci 2001;21(RC143):1–6.
71. Chen JF, Steyn S, Staal R, Petzer JP, Xu K, Van Der Schyf CJ, Castagnoli K, Sonsalla PK, Castagnoli N Jr, Schwarzschild MA. 8-(3Chlorostyryl) caffeine may attenuate MPTP neurotoxicity through dual actions of monoamine oxidase inhibition and A2A receptor antagonism. J Biol Chem 2002;277:36040–4.
72. Aguiar LMV, Hélio V. Nobre Jr Macêdo DS, Oliveira AA, Freitas RM, Vasconcelos SM, Cunha GM, Sousa FC, Viana GS. Neuroprotective effects of caffeine in the model of 6-hydroxydopamine lesion in rats. Pharmacology, Biochemistry and Behavior 2006;84:415–419.
73. DeFeudis F.V. Ginkgo biloba Extract (EGb 761): from Chemistry to the Clinic, Ullstein Medical, Wiesbaden.1998.
74. Rojas P, Serrano-García N, Mares-Sámano JJ, Medina-Campos ON, Pedraza- Chaverri J, Ogren SO. EGb761 protects against nigrostriatal dopaminergic neurotoxicity in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinsonism in mice: role of oxidative stress. Eur J Neurosci 2008;28:41-50.
75. White HL, Scates PW, Cooper BR. Extracts of Ginkgo biloba leaves inhibit monoamine oxydase. Life Sci 1996;58:1315–21.
76. Jiang Y, Tu PF. Xanthone O-glycosides from Polygala tenuifolia. Phytochemistry 2002;60:813–16.
77. Choi JG, Kim HG, Kim MC. Polygalae radix inhibits toxin-induced neuronal death in the Parkinson’s disease models. Journal of Ethnopharmacology 2011;134 414-21.
78. Cheng MC, Li CY, Ko HC, Ko FN, Lin YL, Wu TS. Antidepressant principle of the roots of Polygala tenuifolia. Journal of Natural Products 2006;69:1305–09.
79. Kawashima K, Miyako D, Ishino Y. Antistress effects of 3,4,5-trimethoxycinnamic acid, an active constituent of roots of Polygala tenuifolia (Onji). Biological & Pharmacetical Bulletin. 2004;27:1317–19.
80. Wang JY, Chiu JH, Tsai TH, Tsou AP, Hu CP, Chi CW, Yeh SF, Lui WY, Wu CW, Chou CK. Gene expression profiling predicts liver responses to a herbal remedy after partial hepatectomy in mice. International Journal of Molecular Medicine 2005;16:221–31.
81. Lee HJ, Noh YH, Lee DY, Kim YS. Baicalein attenuates 6-hydroxydopamine- induced neurotoxicity in SH-SY5Y cells. Eur J Cell Biol 2005;84:897-905.
82. Cheng Y, He G, Mu X. Neuroprotective effect of baicalein against MPTP neurotoxicity: Behavioral, biochemical and immunohistochemical profile Neuroscience Letters 2008;441:116–20.
83. Lin YJ, Hou YC, Lin CH, Hsu YA, Sheu JJ, Lai CH, Chen BH, Lee Chao PD, Wan L, Tsai FJ. Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells. Biochem Biophys Res Commun 2009;378:683–88.
84. Lin CM, Lin RD, Chen ST. Neurocytoprotective effects of the bioactive constituents of Pueraria thomsonii in 6-hydroxydopamine (6-OHDA)-treated nerve growth factor (NGF)-differentiated PC12 cells. Phytochemistry. 2010;71:2147-56.
85. Hsieh CL, Chen MF, Li TC. Anticonvulsant effect of Uncaria rhynchophylla (Miq.) Jack. in rats with kainic acid-induced epileptic seizure. American Journal of Chinese Medicine 1999; 27:257–264.
86. Yamanaka E, Kimizuka Y, Aimi N, Sakai S, Haginiwa J. Studies of plants containing indole alkaloids. IX. Quantitative analysis of tertiary alkaloids in various parts of Uncaria rhynchophylla Miq. Yakugaku Zasshi. Journal of the Pharmaceutical Society of Japan 1983;103:1028–33
87. Wang Y, Xu H, Fu Q. Protective effect of resveratrol derived from Polygonum cuspidatum and its liposomal form on nigral cells in Parkinsonian rats. Journal of the Neurological Sciences 2011;304:29–34.
88. Khan MM, Ahmad A, Ishrat T, Khan MB, Hoda MN, Khuwaja G, Raza SS, Khan A, Javed H, Vaibhav K, Islam F. Resveratrol attenuates 6-hydroxydopamine- induced oxidative damage and dopamine depletion in rat model of Parkinson’s disease. Brain Res 2010;1328:139-51.
89. Ernst E. Herbal remedies for anxiety—a systematic review of controlled clinical trials. Phytomedicine 2006;13:205–08.
90. Shobana C, Kumar RR, Sumathi T. Alcoholic Extract of Bacopa monniera Linn. Protects Against 6-Hydroxydopamine-Induced Changes in Behavioral and Biochemical Aspects: A Pilot Study. Cell Mol Neurobiol 2012;32:1099-112.
91. Parkinson Study Group. Effect of deprenyl on the progression of disability in early Parkinson’s disease. N Engl J Med 1989;321:1364–3171
92. Sayre LM. Biochemical mechanism of action of the dopaminergic neurotoxin 1- methyl-4-phenyl-1, 2,3,6-tetrahydropyridine (MPTP). Toxicol Lett 1989;48:121–49.
93. Manyam B. Paralysis agitans and levodopa in ‘‘Ayurveda’’: ancient Indian medical treatise. Mov Disord 1990;5:47–8.
94. Damodaran M, Ramaswamy R. Isolation of L-dopa from the seeds of Mucuna pruriens. Biochem J. 1937;31:2149–451.
95. Kempster PA, Bogetic Z, Secombe JW, Martin HD, Balazs NDH, Wahlqvist ML. Motor effects of broad beans (Vicia fava) in Parkinson’s disease: single dose studies. Asia Pac J Clin Nutr 1993;2:85–9.
96. Katzenschlager R, Evans A, Manson A, Patsalos P N, Ratnaraj N, Watt H, Timmermann L, Van der Giessen R, Lees A J. Mucuna pruriens in Parkinson’s disease: a double blind clinical and pharmacological study. J Neurol Neurosurg Psychiatry 2004;75(12):1672–1677.
97. Poewe WH, Lees AJ, Stern GM. Dystonia in Parkinson’s disease: clinical and pharmacological features. Ann Neurol 1988;23:73–78.
98. Apaydin H, Ertan S, O zekmekci S. Broad bean (vicia fava) – a natural source of L-dopa – prolongs ‘‘on’’ periods in patients with Parkinson’s disease who have ‘‘on-off’’ fluctuations. Mov Disord 2000;15:164–6.
99. Spengos M, Vassilopoulos D. Improvement of Parkinson’s disease after Vicia Faba consumption. Ninth Int Sympo-sium on Parkinson’s disease. Jerusalem, Israel. Abs 1988;46.
100. He SM, Jiang J. Review on TCM treatment of migraine and tension-type headache. ChinArch Tradit Chin Med 2006;24:1469–71.
101. An CP, Cheng W. Review on mechanisms and symptoms of depression in TCM (Traditional Chinese Medicine). Inf Tradit Chin Med 2007;24:12–4.
102. Lee CH, Hwang DS, Kim HG, Oh H, Park H, Cho JH, Lee JM, Jang JB, Lee KS, Oh MS. Protective effect of Cyperi rhizoma against 6-hydroxydopamine-induced neuronal damage. J Med Food 2010;13:564-71.
103. Liu LK, Lee HJ, Shih YW, Chyau CC, Wang CJ. Mulberry anthocyanin extracts inhibit LDL oxidation and macrophage-derived foam cell formation induced by oxidative LDL. J Food Chem 2008;73:113–120.
104. Kim HG, Ju MS, Shim JS, Kim MC, Lee SH, Huh Y, Kim SY, Oh MS. Mulberry fruit protects dopaminergic neurons in toxin-induced Parkinson’s disease models. British Journal of Nutrition 2010;104:8–16.
105. Zheng ZP, Cheng KW, Chao J, Wu J, Wang M. Tyrosinase inhibitors from paper mulberry. Food Chemistry 2008;106:529–35.
106. Manthey JA, Guthrie N, Grohmann K. Biological properties of citrus flavonoids pertaining to cancer and inflammation. Curr Med Chem 2001;8:135-53.
107. Datla KP, Christidou M, Widmer WW, Rooprai HK, Dexter DT. Tissue distribution and neuroprotective effects of citrus flavonoid tangeretin in a rat model of Parkinson’s disease. Neuroreport 2001;12:3871-5.
108. Zbarsky V, Datla KP. Neuroprotective properties of the natural phenolic antioxidants curcumin and naringenin but not quercetin and fisetin in a 6-OHDA model of Parkinson’s disease. Free Radical Research 2005;39:1119-25.
109. Vauzour D, Lee HY, Rodriguez-Mateos A, Williams RJ, Spencer JP. The citrus flavanone naringenin inhibits inflammatory signalling in glial cells and protects against neuroinflammatory injury. Archives of Biochemistry and Biophysics 2009;484:100–09.
110. Fatemeh Eshraghi-Jazi, Alaei H, Azizi-Malekabadi H, Gharavi-Naini M, Pilehvarian A, Ciahmard Z. The effect of red grape juice and exercise, and their combination on parkinsons disease in rats. Avicenna Journal of Phytomedicine 2012;90-9.
111. Bournival J, Quessy P, Martinoli MG. Protective Effects of Resveratrol and Quercetin Against MPP+-Induced Oxidative Stress Act by Modulating Markersof Apoptotic Death in Dopaminergic Neurons. Cell Mol Neurobiol 2009;29:1169–1180.
112. Chao J, Yu MS, Ho YS, Wang M, Chang RC. Dietary oxyresveratrol prevents Parkinsonian mimetic 6-hydroxydopamine neurotoxicity. Free Radical Biology and Medicine 2008;45:1019–26.
113. Kim KM, Chun SB, Koo MS, Choi WJ, Kim TW, Kwon YG, Chung HT, Billiar TR, Kim YM. Differential regulation of NO availability from macrophages and endothelial cells by the garlic component S-allyl cysteine. Free Radic Biol Med 2001;30:747–56.
114. Garcia NS, Medina-Campos ON, Pedraza-Chaverri J. S-Allylcysteine, a garlic compound, protects against oxidative stress in1-methyl-4-phenylpyridinium- induced parkinsonism in mice. Journal of nutritional biochemistry 2011;934-944.
115. Tachakittirungrod S, Ikegami F. Antioxidant Active Principles Isolated from Psidium guajava Grown in Thailand. Scientia Pharmaceutica (Sci. Pharm.) 2007;75:179-93.
116. Zhang ZT, Cao XB, Nian Xiong N, Wang HC, Huang JS, Sun SG, Wang T. Morin exerts neuroprotective actions in Parkinson disease models in vitro and in vivo. Acta Pharmacologica Sinica 2010;31:900–06.
117. Suganuma H, Hirano T, Arimoto Y, Inakuma TJ. Effect of tomato intake on striatal monoamine level in a mouse model of experimental Parkinson’s disease. J Nutr Sci Vitaminol 2002;3:251-4.
118. Radad K, Moldzio R, Taha M, Rausch WD. Thymoquinone protects dopaminergic neurons against MPP+ and rotenone. Phytother Res 2009;23:696- 700.
119. Hemalatha S, Raghunath M, Ghafoorunissa. Dietary sesame oils inhibits iron- induced oxidative stress in rats [corrected]. Br J Nutr 2004;92:581-7.
120. Jeng KC, Hou RC, Wang JC, Ping LI. Sesamin inhibits lipopolysaccharide-induced cytokine production by suppression of p38 mitogen-activated protein kinase and nuclear factor-kappaB. Immunol Lett 2005;97:101-6.
121. Lahaie-Collins V, Bournival J, Plouffe M, Carange J, Martinoli MG. Sesamin modulates tyrosine hydroxylase, superoxide dismutase, catalase, inducible NO synthase and interleukin-6 expression in dopaminergic cells under MPP+ – induced oxidative stress. Oxid Med Cell Longev 2008;1:54-62.
122. Rao CV, Rivenson A, Simi B, Reddy BS. Chemoprevention of colon carcinogenesis by dietary curcumin, a naturally occurring plant phenolic compound. Cancer Res 1995;55:259–266.
123. Vajragupta O, Boonchoong P, Watanabe H, Tohda M, Kummasud N, Sumanont Y. Manganese complexes of curcumin and its derivatives: Evaluation for the radical scavenging ability and neuroprotective activity. Free Radical Biology & Medicine 2003;35:1632–44.
124. Rajeswari A, Sabesan M. Inhibition of monoamine oxidase-B bythe polyphenolic compound, curcumin and its metabolite tetrahydrocurcumin, in a model of Parkinson’s disease induced by MPTP neurodegeneration in mice. Inflammopharmacology 2008;16:96–99.
125. Ojha RP, Rastogi M, Devi BP, Agrawal A, Dubey GP. Neuroprotective Effect of Curcuminoids Against Inflammation-Mediated Dopaminergic Neurodegeneration in the MPTP Model of Parkinson’s Disease. J Neuroimmune Pharmacol 2012;21[Epub ahead of print] .
126. PandeyN, Strider J, Nolan WC, Yan SX, Galvin JE. Curcumin inhibits aggregation of alpha-synuclein. Acta Neuropathol 2008;115:479–89.
127. Yu S, Zheng W, Xin N, Chi ZH, Wang NQ, Nie YX, Feng WY, Wang ZY. Curcumin prevents dopaminergic neuronal death through inhibition of the c-Jun Nterminal kinase pathway. Rejuvenation Res 2010;13:55–64.
128. Kabuto H, Nishizawa M, Tada M, Higashio C, Shishibori T, Kohno M. Zingerone [4-(4-hydroxy-3-methoxyphenyl)-2-butanone] prevents 6-hydroxydopamine- induced dopamine depression in mouse striatum and increases superoxide scavenging activity in serum. Neurochem Res. 2005;30:325-32.
129. Kyung KS, Gon JH, Geun KY, Sup JJ, Suk WJ, Ho KJ. 6Shogaol, a natural product, reduces cell death and restores motor function in rat spinal cord injury. Eur J Neurosci 2006;24:1042-52.
130. Kabuto H, Tada M, Kohno M. Eugenol [2-methoxy-4-(2propenyl) phenol] prevents 6-hydroxydopamine-induced dopamine. Biol Pharm Bull. 2007:30:423-7.
131. Liu DZ, Xie KQ, Ji XQ, Ye Y, Jiang CL, Zhu XZ. Neuroprotective effect of paeoniflorin on cerebral ischemic rat by activating adenosine A receptor in a different manner from its classical agonists. Br J Pharmacol 2005;146: 604–11.
132. Liu HQ, Zhang WY. Paeoniflorin attenuates neuroinflammation and dopaminergic neurodegeneration in the MPTP model of Parkinson’s disease by activation of adenosine A1receptor. British Journal of Pharmacology 2006;148:314–25.
133. Zhang X, Xu JK, Wang J, Wang NL, Kurihara H, Kitanaka S, Yao XS. Bioactive bibenzyl derivatives and fluorenones from Dendrobium nobile. J Nat Prod 2007;70:24–28.
134. Song JX, Shaw PC. Chrysotoxine, a novel bibenzyl compound, inhibits 6- hydroxydopamine induced apoptosis in SH-SY5Y cells via mitochondria protection and NF-κB modulation. Neurochemistry International 2010;57:676–89.
135. Park SE, Kim S, Sapkota K, Kim SJ. Neuroprotective effect of Rosmarinus officinalis extract on human dopaminergic cell line, SH-SY5Y. Cell Mol Neurobiol 2010;30(5):759-67.
136. Wang J, Xu H, Jiang H, Du X, Sun P. Neurorescue effect of rosmarinic acid on 6- hydroxydopamine-lesioned nigral dopamine neurons in rat model of Parkinson’s disease. J Mol Neurosci 2012;47:113-9.
137. Kim SJ, Kim JS, Cho HS, Lee HJ, Kim SY, Kim S, Lee SY, Chun HS. Carnosol, a component of rosemary (Rosmarinus officinalis L.) protects nigral dopaminergic neuronal cells. Neuroreport 2006;17:1729-33.
138. Geng X, Song L, Pu X, Tu P. Neuroprotective effects of phenylethanoid glycosides from Cistanches salsa against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic toxicity in C57 mice. Biol Pharm Bull 2004; 27(6):797-801.
139. Li WW, Yang R, Cai DF. Protective effects of Cistanche total glycosides on dopaminergic neuron in substantia nigra of model mice of Parkinson’s disease. Zhongguo Zhong Xi Yi Jie He Za Zhi 2008;28:248-51.
140. Hua An, In Su Kim, Park PJ, Lim BO, Choi WS, Lee KH, Choi DK. Protective effects of Gastrodia elata Blume on MPP+-induced cyto toxicity in human dopaminergic SH-SY5Y cells. Journal of Ethnopharmacology 2010;130:290–298.
141. Kim IS, Choi DK, Jung HJ. Neuroprotective effects of vanillyl alcohol in Gastrodia elata Blume through suppression of oxidative stress and anti-apoptotic activity in toxin-induced dopaminergic MN9D cells. Molecules 2011;16:5349-61.
142. Zhang R, Xu S, Cai Y, Zhou M, Zuo X, Chan P. Ganoderma lucidum Protects Dopaminergic Neuron Degeneration through Inhibition of Microglial Activation. Evidence-Based Complementary and Alternative Medicine 2011;Article ID 156810, 9 pages doi:10.1093/ecam/nep075.
143. Ahlskog JE, Muenter MD. Frequency of levodopa-related dyskinesias and motor fluctuations as estimated from the cumulative literature. Movement Disorders 2000;16: 448–458.
144. Rao SS, Hofmann LA, Shakil A. Parkinson’s disease: diagnosis and treatment. American Family Physician 2006;74: 2046–2054.
145. Schulz KF, Altman DG, Moher D. CONSORT 2010 statement: Updated guidelines for reporting parallel group randomised trials. Journal of Pharmacology and Pharmacotherapeutics 2010;1: 100–107.
146. Ross JS, Mulvey GK, Hines EM, Nissen SE, Krumholz HM. Trial publication after registration in ClinicalTrials.Gov: a cross-sectional analysis. PLoS Med 2009;6: e1000144.