C. Arganini1, L.M. Donini2, M. Peparaio1, F. Sinesio1
1. CRA-NUT Research Centre on Food and Nutrition, Roma, Italy; 2. Sapienza University of Rome, Experimental Medicine Department, Roma, Italy
Corresponding Author: C. Arganini, CRA-NUT Research Centre on Food and Nutrition, Via Ardeatina, 546, 00178 Roma, Italy, E-mail: firstname.lastname@example.org
Background: Age-related chemosensory impairments (i.e., reduction of taste and smell acuity) affect a large proportion of the population older than 65 years. These conditions can affect overall health, quality of life and influence food appreciation and intake. The knowledge about the relation between chemosensory perception and hedonic food liking in older people might facilitate the development of effective strategies aiming to improve their nutritional wellbeing. Objectives: The key objective of this study is to test the influence of a sensory compensatory strategy on liking and intake of a functional red fruit drink in undernourished nursing home elderly people. Design: The research consisted of three main phases. In the first step the enrolled subjects were classified on the basis of their taste and olfaction sensitivity; in the second step the sensory compensatory strategy, aiming to counteract age-associated sensory losses and increase product pleasantness, was identified; the last step consisted of 14 days of exposure to each drink variant (standard and enhanced) to observe the effects of sensory modifications on liking and intake. Setting: “Villa delle Querce” Clinical Rehabilitation Institute of Nemi (Rome- Italy). Participants: 76 elderly people (64-97 years). Results: Overall the prevalence of measured chemosensory impairments in this sample of institutionalized older adults was very high (81%). The sensory modification strategy did influence significantly neither the intake nor liking in the elderly people. Consistent with the findings of previous studies (3, 6, 24, 25) no significant correlation was found between sensory capabilities and hedonic responses. Conclusion: These findings highlight that, in accordance to other studies (2, 3, 6-8), the role of sensory compensatory strategies in increasing products appreciation and intake of institutionalized elderly people have been overestimated.
Key words: Chemosensory impairment, sensory compensatory strategy, undernutrition, institutionalized elderly people.
In institutionalized elderly people poor energy intake is an important factor related to malnutrition which is often associated with frailty and increased morbidity and mortality (1). One of the potential causes of inadequate food intake is considered the age related reduction of taste and smell acuity (2). Several studies have found a progressive decline in taste and smell functioning, which tends to begin around 65 years of age (3, 4). The prevalence of sensory impairment is reported to be high and to increase with age (5-8).The causes can be physiological aging as well as certain disease states, pharmacologic and surgical interventions, radiation and environmental exposure (4). Chemosensory impairments are supposed to affect food perception and liking and consequently to modify food choice, although data in support of this « sequence of assumptions » are currently lacking (9, 10).
Chemosensory deficit experienced by elderly people generally cannot be reversed. However, sensory interventions including intensification of food taste and odor might compensate for reduced acuity. Food flavor enhancement strategies for undernourished patients have been used over the last 25 years to increase intake of nutrient dense food (11) with contradictory results (3, 6- 8). According to some studies (11-13) addition of flavor could be an efficient strategy to cope with nutritional problems such as “anorexia of aging”. However, as pointed out by Kremer et al (8) in these earlier studies poor sensory acuity of elderly people has been assumed but not measured. Previous studies (12, 14) suggested that higher liking for stronger flavor and taste is an age- related feature, which might be attributed to a compensation effect. Some authors suggested that elderly people may prefer a higher sweetness intensity (14). There are, however, other researches (3, 15) that are in contradiction with these findings. Also, in most hedonic studies, liking is based on first impressions of an evaluated product, and does not reflect preferences that result after a longer exposure to the product.
In the light of the above considerations, the aim of the present study is to observe the effect of a sensory compensatory strategy on the intake and hedonic response to a functional drink in undernourished elderly patients living in nursing home, classified on the basis of their taste and smell sensitivity compared to a group of healthy free living adults with normal chemosensory acuity.
The research consisted of three main phases. In the first step the enrolled subjects were classified on the basis of their taste and olfaction sensitivity; in the second step the sensory compensatory strategy, aiming to counteract age- associated sensory losses and increase product pleasantness, was developed; while, the last step consisted of 14 days of exposure to each drink variant (standard and enhanced) to observe the effects of sensory modifications on liking and intake.
Materials and Methods
Residents from “Villa delle Querce” Clinical Rehabilitation Institute of Nemi (Rome, Italy) participated in this study. The local ethics committee approved the study.
A clinical screening was performed and the followings were evaluated for each individual: clinical status, number of pathologies, number of medications taken, cognitive functions (by Short Portable Mental Status Questionnaire, SPMSQ), nutritional status (with Mini Nutritional Assessment, MNA).
Inclusion criteria were: age >65 years; being at risk of malnutrition (MNA ≤23. 5); energy deficiency (intake of 20 kcal/kg/day or lower); no severe cognitive impairment (SPMSQ
Participants were selected for their physiological and cognitive ability to carry out the evaluation. After the screening 76 subjects were included in the study and provided
their written informed consent. The mean age of the sample was 81.4 ±8.3 years (age range 64-97 years). Seventy percent participants were women. Prevalence of chemosensory impairments was measured on 76 individuals. Eleven participants dropped out because of taste dislike to the drink, health and personal reasons.
A group of adult (n=38; 30-50 years) with normal sensory acuity, recruited from the staff of CRA-NUT served as control.
Chemosensory acuity was assessed by means of three validated sensitivity tests produced by Burghart (GmbH Wedel Germany). The original methodology of test administration (16, 17) was simplified in order to make it more suitable for elderly people (18, 19).
Testing for odor identification. This method is based on pen-like odor dispensing devices. Individuals were presented with 9 pens containing common odorants at an interval of 30 seconds. They were asked to smell each pen and to choose the odor name from four alternatives. For instance, whether it was presented orange odor, subjects had to choose between orange, strawberry, blackberry, pineapple.
Threshold odor test. Odor thresholds for phenylethanol were assessed using a single-staircase, three alternative forced choice (3- AFC) procedure. Three pens were presented in a randomized order, with two containing the solvent and the third the odorant. Subjects had to identify the odor-containing pen. Twelve triplets were presented at intervals of approximately 20 s.
Gustatory test . The used technique is based on strips made from filter paper which were impregnated with different taste solutions (sweet, sour, salty and bitter). Three concentrations were used for each taste quality resulting in a maximum total score of 12. The taste strips were presented in increasing concentrations in a randomized order and placed on the tongue. Then subjects were asked to close the mouth and choose one of five possible answers on a form (sweet, sour, salty, bitter, no taste).
Subjects were then classified in four groups on the basis of the performance in the screening test: 1) Normal chemosensory acuity/function; 2) Taste impaired (gustatory test
Subjects affected by anosmia and ageusia were excluded from successive evaluations.
Sensory compensatory strategy
The product employed is a functional red fruit (grapes, raspberries and red berry) drink containing antioxidant ingredients (Vitamin C, vitamin E and green tea extract), supplied by Barilla Food Company. The sensory compensatory strategy aiming to make the drink more palatable for elderly people, was developed with the aid of our expert sensory panel (n=8). Following the results of the sensory analysis, the manufacturer provided two versions of the functional drink: the standard version (red fruit drink commercially available) and the enhanced version with a significantly (p
Each subject was randomly assigned to consume 1 glass (125 ml) of one drink variant (enhanced or standard) along with breakfast for 14 consecutive days and, after 1 day washout period, switched to the other variant for 14 days. The subjects of the control group were asked to consume the functional drinks along with breakfast at their own home. The effects of exposure to the two variants of the drink on intake and hedonic response were assessed during the experimental period. Participants were asked to rate the “Liking “of the drink on the 1st , 6h and 14th day of each treatment with a 7 point Likert Scale. The left anchor of the scale was explained as “I don’t like it at all” and the right anchor corresponded with “I like it very much”. The intake was measured daily, only on the elderly subjects, on the basis of leftover.
All statistical analyses were performed using XLSTAT v. 2012.1.01 (Addinsoft).
The distribution of the prevalence of chemosensory impairment by gender was tested using Chi-square analysis and Fisher’s exact test.
Data on hedonic ratings were submitted to the analysis of variance (ANOVA) to test the effect of drink samples (standard, sensory enhanced), subject clusters (C= control group; N= elderly with no sensory deficit; S= elderly with smell impairment; T= elderly with taste impairment; TS= elderly with taste and smell impairment), sequence of drink sample administration and time (1, 6, 14 days of treatment). The two-way interactions were also analyzed.
One-way ANOVA and Fisher’s least significant difference were performed for mean separation.
Prevalence of chemosensory impairment
Overall the prevalence of measured chemosensory impairment in this sample of institutionalized elderly people was very high (81%).
Table 1 shows in details the prevalence of taste and smell impairment for the whole sample and by gender. The distribution of chemosensory impairment did not significantly differ for gender (P=0.656). Measured taste impairment is the same among men and women, while smell impairment tended to be higher in men. The observed very high prevalence of taste impairment is probably related to the intake of large number of medications, as polypharmacy is considered a common cause of impaired chemosensory perception (20).
The daily intake was measured registering the amount of leftover in the glass. The scale was from 0 to 4, where 0 means no leftover; 1= 1 /4 of the glass; 2= half glass; 3= ¾ glass, and 4= full glass. A high variability of leftover was observed. No effect of sample (standard or sensory enhanced) on intake (P=0.980) was observed. No significant difference among the groups of subjects (P= 0.974) was detected. Average intake was 70% of sample both for standard and enhanced variant. Intake was stable over time.
Liking was measured on 67 elderly (11 drop-out) grouped by their taste and olfactory sensitivity and on 38 adults with normal sensory acuity (control group).
According to the ANOVA results, the compensatory strategy did not lead to an increase of the product liking among the elderly. From the analysis of variance it emerged that overall the elderly people rated both drink variants more positively then the control group (Patarax without prescription(P=0.447). No significant interactions were found.
In table 2 the hedonic ratings of the different groups of subjects for the standard sample at day 1, 6 and 14 of treatment are reported. Liking is significantly lower in the control group and in elderly people with normal sensory acuity at the beginning (day 1) of the treatment (P= 0.052).
A lightly downward trend in liking over time was observed for the standard sample in the control group (P = 0.073) while a significant increase among the group of elderly with normal sensory acuity (P =0.042). In the groups of elderly with sensory deficit the liking doesn’t change over time.
In table 3 the hedonic ratings for the enhanced sample of the different groups of subjects are reported. Overall the hedonic response to the enhanced variant does not significantly change in any group after 14 days repeated exposure. The four subgroups of elderly rated the enhanced drink more positively then the control group (P
In the rows means with different letter are statistically different; 1. Lower than S, T, TS at p
1. Lower than N, S, T, TS at p
Prevalence of chemosensory impairment
The prevalence of measured chemosensory impairments in this study was quite high. Specifically, the frequency of smell impairment in this population of frail elderly people was comparable to data obtained in previous studies (2, 5), while the frequency of taste impairment was higher than expected on the basis of previous investigations, pointing out that aging seems to affect smell stronger then taste (6-8). This could be related to the health condition and large use of medications (20) in the sample considered in the present study. Moreover, overall men seem to have higher prevalence of chemosensory impairment as reported in other studies (5, 21).
Intake and hedonic responses
The intake of both drinks was consistent over time in all groups of subjects and was not influenced by the sensory modification. So the compensatory strategy was not proven to be effective in increasing the intake of the drink. The general consistency of hedonic ratings over time indicates that both drink variants were equally accepted among the elderly subjects.
Overall, as shown in other researches (3, 22) the elderly subjects rated the samples more positively than the adult group. This result can be due to the perceived health benefits of the drink or to the novelty respect to a monotonous diet at the nursing home, although should also be considered as a relevant factor the willingness of the elderly people to please the interviewer.
Consistently with the findings of previous studies (3, 6, 23, 24) no significant correlation was found between sensory capabilities and hedonic responses in elderly people. Here subjects with taste impairments rated more positively the enhanced sample, but as the relation is not significant and the size of the subgroups in this study is small, and further investigation is needed to explore the association.
This research does not support the assumption that age associated decline in chemosensory acuity inevitably reduces food liking and intake. In accordance with other studies (2, 3, 6-8) it can be concluded that the role of sensory compensatory strategies in increasing products appreciation and intake in frail elderly people has been overestimated. Therefore, in the light of the results of this study it can be suggested that more effective strategies to increase product pleasantness and intake for institutionalized elderly people should definitely take into consideration other age-related factor such as psychological conditions, loneliness, reduced social interaction and limited food choice.
Acknowledgements: The authors wish to thank Dr. Barbara Neri, Dr Edda Cava, the staff and the residents of “Villa delle Querce”
Clinical Rehabilitation Institute of Nemi (Rome- Italy)
Funding: This work was supported by a grant from the Italian Ministry of Agricultural, Food and Forestry Policies (Project Qualifu-Alieta). The products were supplied by Barilla Food Company.
1. Donini LM, De Felice MR, Savina C, Coletti C, Paolini M, Laviano A, Scavone L, Neri B, Cannella C. Predicting the outcome of long-term care by clinical and functional indices: the role of nutritional status. J Nutr Health Aging 2011; 15 (7):586-592
2. Essed NE, Van Staveren WA, Kok FJ, Graaf C. No effect of 16 weeks flavor enhancement on dietary intake and nutritional status of nursing home elderly Original Research Article. Appetite 2007; 48(1): 29-36.
3. Koskinen S, Kalviainen N, & Tuorila H. Flavor enhancement as a tool for increasing pleasantness and intake of a snack-product among the elderly. Appetite 2003; 41: 87–96.
4. Schiffman SS. Intensification of sensory properties of foods for the elderly. J Nutr 2000; 130:S927–S930.
5. Murphy C, Schubert CR, Cruickshanks KJ, Klein BEK, Klein R, Nondahl DM. Prevalence of olfactory impairment in older adults. JAMA 2002; 288(18):2307–2312.
6. Kremer S, Bult JHF, Mojet J, Kroeze JHA. Food perception with age and its relationship to pleasantness. Chem Senses 2007; 32: 591-602.
7. Kremer S, Bult JHF, Mojet J, Kroeze JHA. Compensation for age-associated chemosensory losses and its effect on the pleasantness of a custard dessert and a tomato drink. Appetite 2007; 48:96-103
8. Kremer S, Mojet J, Kroeze JHA. Differences in perception of sweet and savoury waffles between elderly and young subjects. Food Qual Pref 2007; 18:106-116.
9. Mattes RD. The chemical senses and nutrition in aging: Challenging old assumptions. J Am Diet Assoc 2002;102: 192–196.
10. Laureati M, Pagliarini E, Calcinoni O, Bidoglio M. Sensory acceptability of traditional food preparations by elderly people. Food Qual Pref 2006; 17:43- 52.
11. Shiffman SS. Taste and smell losses in normal aging and disease. JAMA 1997; 278:1357-1362.
12. Griep, MI, Mets T F & Massart DL. Effects of flavor amplification of Quorns and yoghurt on food preference and consumption in relation to age. BMI and odour perception. Br J Nutr 2000;83: 105–113.
13. Mathey M-FAM, Siebelink E, De Graaf C, Van Staveren WA. Flavor enhancement of food improves dietary intake and nutritional status of elderly nursing home residents. J Gerontol 2001;56: M200–M205.
14. De Jong N, De Graaf, C, Van Staveren WA. Effect of sucrose in breakfast items on pleasantness and food intake in the elderly. Physiol Behav 1996;60:1453–1462.
15. Mojet J, Christ-Hazelhof E, Heidema J. Taste perception with age: pleasantness and its relationships with threshold sensitivity and suprathreshold intensity of five taste qualities. Food Qual Pref 2005;16:413–423.
16. Hummel T, Sekinger B, Wolf S, Pauli E, Kobal G. ‘‘Sniffin’ Sticks’’: olfactory performance assessed by the combined testing of odor identification, odor discrimination and olfactory threshold. Chem Senses 1997; 22:39–52.
17. Mueller C, Kallert S, Renner B, Stiassny K, Temmel AFP, Hummel T, Kobal G. Quantitative assessment of gustatory function in a clinical context using impregnated “taste strips” made from filter paper. Rhinology 2003;41 (1): 2-6.
18. Sinesio F, Arganini C , Moneta E, Peparaio M, Comendador FJ. Sensory compensatory strategy to increase functional foods liking and intake in undernourished elderly institutionalised subjects.II World Congress of Public Health Nutrition, Porto 23-25 September 2010.
19. Arganini C and Sinesio F. Effects of Sensory Compensatory Strategy on Hedonic Responses to a Functional Beverage in Undernourished Institutionalised Elderly:preliminary results. 9th Pangborn Sensory Science Symposium, Toronto, 4-8 September 2011.
20. Bromley SM. Smell and Taste Disorders: A Primary Care Approach. Am Fam Physician 2000; 61(2):427-436.
21. Bramerson A, Johansson L, Ek L, Nordin S, Bende M. Prevalence of olfactory dysfunction: The Skovde Population-Based Study. Laryngoscope 2004; 114:733–737.
22. Pelchat, ML. You can teach an old dog new tricks: olfaction and responses to novel foods by the elderly. Appetite 2000; 35:153–160.
23. Issanchou S. Changing food liking with ageing. Food Qual Pref 2004; 15:908–909.
24. Forde CG, Delahunty CM. Understanding the role cross-modal sensory interactions play in food acceptability in younger and older consumers. Food Qual Pref 2004; 15:715–727.